Fluorine in PDB 7x24: Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State

All present enzymatic activity of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State:
7.2.2.13; 7.2.2.19;

The structure of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State also contains other interesting chemical elements:

Potassium	(K)	3 atoms
Aluminium	(Al)	1 atom
Magnesium	(Mg)	1 atom

The binding sites of Fluorine atom in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State (pdb code 7x24). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 4 binding sites of Fluorine where determined in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State, PDB code: 7x24:
Jump to Fluorine binding site number: 1; 2; 3; 4;

Fluorine binding site 1 out of 4 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:F1111 b:17.5 occ:1.00 F1 A:ALF1111 0.0 17.5 1.0 AL A:ALF1111 1.8 17.5 1.0 MG A:MG1112 2.1 17.8 1.0 OD2 A:ASP388 2.3 17.1 1.0 F3 A:ALF1111 2.5 17.5 1.0 F4 A:ALF1111 2.6 17.5 1.0 CG A:ASP388 3.0 17.1 1.0 OD1 A:ASP388 3.2 17.1 1.0 OD2 A:ASP733 3.3 16.0 1.0 F2 A:ALF1111 3.6 17.5 1.0 OD1 A:ASN732 3.6 18.3 1.0 ND2 A:ASN732 3.7 18.3 1.0 CA A:GLY232 3.8 24.7 1.0 O A:THR390 4.0 14.5 1.0 OD1 A:ASP729 4.0 12.9 1.0 CG A:ASN732 4.1 18.3 1.0 CG A:ASP733 4.1 16.0 1.0 CB A:ASP388 4.2 17.1 1.0 OD1 A:ASP733 4.3 16.0 1.0 O A:GLY232 4.3 24.7 1.0 C A:GLY232 4.6 24.7 1.0 CA A:GLY730 4.6 16.4 1.0 N A:GLY730 4.7 16.4 1.0 O A:THR231 4.8 23.0 1.0 N A:GLY232 4.8 24.7 1.0

Fluorine binding site 2 out of 4 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:F1111 b:17.5 occ:1.00 F2 A:ALF1111 0.0 17.5 1.0 AL A:ALF1111 1.8 17.5 1.0 F4 A:ALF1111 2.5 17.5 1.0 F3 A:ALF1111 2.5 17.5 1.0 OG1 A:THR629 2.8 14.9 1.0 OD1 A:ASP388 2.8 17.1 1.0 N A:GLY630 3.0 17.1 1.0 O A:THR231 3.5 23.0 1.0 F1 A:ALF1111 3.6 17.5 1.0 OE1 A:GLU233 3.7 30.4 1.0 CA A:THR629 3.7 14.9 1.0 CB A:THR629 3.8 14.9 1.0 CG A:ASP388 3.8 17.1 1.0 CA A:GLY630 3.9 17.1 1.0 C A:THR629 3.9 14.9 1.0 OD2 A:ASP388 4.2 17.1 1.0 N A:ASP631 4.3 20.8 1.0 CD A:GLU233 4.3 30.4 1.0 C A:GLY630 4.4 17.1 1.0 N A:THR390 4.4 14.5 1.0 ND2 A:ASN732 4.5 18.3 1.0 OG1 A:THR390 4.5 14.5 1.0 CA A:GLY232 4.6 24.7 1.0 MG A:MG1112 4.6 17.8 1.0 C A:THR231 4.6 23.0 1.0 N A:LYS389 4.6 15.1 1.0 CB A:THR390 4.7 14.5 1.0 OE2 A:GLU233 4.7 30.4 1.0 NZ A:LYS710 4.8 16.1 1.0 C A:GLY232 4.9 24.7 1.0 CB A:LYS389 5.0 15.1 1.0 O A:GLY232 5.0 24.7 1.0

Fluorine binding site 3 out of 4 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:F1111 b:17.5 occ:1.00 F3 A:ALF1111 0.0 17.5 1.0 AL A:ALF1111 1.8 17.5 1.0 F1 A:ALF1111 2.5 17.5 1.0 F2 A:ALF1111 2.5 17.5 1.0 MG A:MG1112 2.6 17.8 1.0 O A:GLY232 2.9 24.7 1.0 CB A:THR390 3.2 14.5 1.0 C A:GLY232 3.3 24.7 1.0 O A:THR390 3.4 14.5 1.0 OD2 A:ASP388 3.4 17.1 1.0 CA A:GLY232 3.4 24.7 1.0 OD1 A:ASP388 3.5 17.1 1.0 F4 A:ALF1111 3.6 17.5 1.0 OE2 A:GLU233 3.6 30.4 1.0 OG1 A:THR390 3.8 14.5 1.0 N A:THR390 3.8 14.5 1.0 CG A:ASP388 3.8 17.1 1.0 CA A:THR390 3.8 14.5 1.0 CD A:GLU233 3.9 30.4 1.0 OE1 A:GLU233 3.9 30.4 1.0 C A:THR390 4.0 14.5 1.0 O A:THR231 4.0 23.0 1.0 CG2 A:THR390 4.2 14.5 1.0 N A:GLU233 4.4 30.4 1.0 N A:GLY232 4.5 24.7 1.0 C A:THR231 4.7 23.0 1.0 OG1 A:THR629 4.8 14.9 1.0 CG A:GLU233 4.9 30.4 1.0

Fluorine binding site 4 out of 4 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 Spwc Mutant in (2K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:F1111 b:17.5 occ:1.00 F4 A:ALF1111 0.0 17.5 1.0 AL A:ALF1111 1.8 17.5 1.0 OD1 A:ASP388 2.5 17.1 1.0 F2 A:ALF1111 2.5 17.5 1.0 F1 A:ALF1111 2.6 17.5 1.0 NZ A:LYS710 2.8 16.1 1.0 ND2 A:ASN732 2.9 18.3 1.0 CG A:ASP388 3.0 17.1 1.0 OD2 A:ASP388 3.4 17.1 1.0 CE A:LYS710 3.4 16.1 1.0 OD1 A:ASP733 3.4 16.0 1.0 F3 A:ALF1111 3.6 17.5 1.0 OD2 A:ASP733 3.9 16.0 1.0 CG A:ASN732 3.9 18.3 1.0 CB A:ASP388 4.0 17.1 1.0 CG A:ASP733 4.1 16.0 1.0 N A:GLY630 4.2 17.1 1.0 OD1 A:ASN732 4.2 18.3 1.0 MG A:MG1112 4.3 17.8 1.0 O A:THR231 4.4 23.0 1.0 O A:VAL628 4.4 16.6 1.0 CA A:THR629 4.4 14.9 1.0 CD A:LYS710 4.7 16.1 1.0 OG1 A:THR629 4.7 14.9 1.0 CA A:ASP388 4.7 17.1 1.0 C A:THR629 4.8 14.9 1.0 CA A:GLY232 4.9 24.7 1.0

V.C.Young, H.Nakanishi, D.J.Meyer, T.Nishizawa, A.Oshima, P.Artigas, K.Abe. Structure and Function of H + /K + Pump Mutants Reveal Na + /K + Pump Mechanisms. Nat Commun V. 13 5270 2022.
ISSN: ESSN 2041-1723
PubMed: 36085139
DOI: 10.1038/S41467-022-32793-0