Fluorine in PDB 2e9t: Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp

All present enzymatic activity of Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp:
2.7.7.48;

The structure of Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp, PDB code: 2e9t was solved by C.Ferrer-Orta, A.Arias, R.Perez-Luque, C.Escarmis, E.Domingo, N.Verdaguer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.60
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	95.597, 95.597, 201.155, 90.00, 90.00, 120.00
R / Rfree (%)	23.3 / 28.9

The structure of Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Fluorine atom in the Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp (pdb code 2e9t). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 2 binding sites of Fluorine where determined in the Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp, PDB code: 2e9t:
Jump to Fluorine binding site number: 1; 2;

Fluorine binding site 1 out of 2 in the Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp within 5.0Å range: probe atom residue distance (Å) B Occ C:F921 b:20.0 occ:0.80 F5 C:5FU921 0.0 20.0 0.8 C5 C:5FU921 1.4 20.0 0.8 C6 C:5FU921 2.3 20.0 0.8 C4 C:5FU921 2.4 20.0 0.8 O4 C:5FU921 2.7 20.0 0.8 C5 C:C920 3.4 44.3 0.8 C4 C:C920 3.5 45.3 0.8 C6 C:C920 3.5 44.5 0.8 N1 C:5FU921 3.6 20.0 0.8 N3 C:5FU921 3.6 20.0 0.8 N3 C:C920 3.6 44.2 0.8 N1 C:C920 3.7 43.1 0.8 C2 C:C920 3.7 43.0 0.8 O2P C:5FU921 3.9 45.0 0.8 C2 C:5FU921 4.1 20.0 0.8 N4 C:C920 4.1 43.8 0.8 C3' C:C920 4.3 44.1 0.8 C2' C:C920 4.3 44.0 0.8 O5' C:5FU921 4.3 44.0 0.8 O2 C:C920 4.5 42.9 0.8 C1' C:C920 4.5 44.7 0.8 P C:5FU921 4.7 45.0 0.8 C1' C:5FU921 4.8 42.9 0.8 O4' C:5FU921 4.8 45.5 0.8 O5' C:C920 4.9 42.4 0.8 NH2 A:ARG179 4.9 39.1 1.0

Fluorine binding site 2 out of 2 in the Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Foot-and-Mouth Disease Virus Rna-Polymerase Rna Dependent in Complex with A Template-Primer Rna and 5F-Utp within 5.0Å range: probe atom residue distance (Å) B Occ F:F921 b:20.0 occ:0.80 F5 F:5FU921 0.0 20.0 0.8 C5 F:5FU921 1.4 20.0 0.8 C6 F:5FU921 2.3 20.0 0.8 C4 F:5FU921 2.4 20.0 0.8 O4 F:5FU921 2.7 20.0 0.8 C4 F:C920 3.6 42.6 0.8 N1 F:5FU921 3.6 20.0 0.8 N3 F:5FU921 3.6 20.0 0.8 C5 F:C920 3.7 42.6 0.8 N3 F:C920 3.7 42.8 0.8 C6 F:C920 3.8 42.1 0.8 C2 F:C920 3.9 42.6 0.8 O2P F:5FU921 3.9 49.0 0.8 N1 F:C920 3.9 43.2 0.8 C2 F:5FU921 4.1 20.0 0.8 N4 F:C920 4.1 41.6 0.8 C3' F:C920 4.3 45.0 0.8 O5' F:5FU921 4.4 48.7 0.8 C2' F:C920 4.4 45.1 0.8 O2 F:C920 4.5 41.3 0.8 P F:5FU921 4.7 50.7 0.8 C1' F:5FU921 4.7 52.1 0.8 C1' F:C920 4.8 43.7 0.8 O4' F:5FU921 4.9 52.8 0.8 NH2 D:ARG179 4.9 39.1 1.0

C.Ferrer-Orta, A.Arias, R.Perez-Luque, C.Escarmis, E.Domingo, N.Verdaguer. Sequential Structures Provide Insights Into the Fidelity of Rna Replication Proc.Natl.Acad.Sci.Usa V. 104 9463 2007.
ISSN: ISSN 0027-8424
PubMed: 17517631
DOI: 10.1073/PNAS.0700518104