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Fluorine in PDB 2zjy: Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp

Enzymatic activity of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp

All present enzymatic activity of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp:
3.6.5.1;

Protein crystallography data

The structure of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp, PDB code: 2zjy was solved by T.Morikawa, A.Muroya, S.Sugio, K.Wakamatsu, T.Kohno, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 28.78 / 2.80
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 79.375, 79.375, 105.297, 90.00, 90.00, 120.00
R / Rfree (%) 18.1 / 26

Other elements in 2zjy:

The structure of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp also contains other interesting chemical elements:

Magnesium (Mg) 1 atom
Aluminium (Al) 1 atom

Fluorine Binding Sites:

The binding sites of Fluorine atom in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp (pdb code 2zjy). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 4 binding sites of Fluorine where determined in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp, PDB code: 2zjy:
Jump to Fluorine binding site number: 1; 2; 3; 4;

Fluorine binding site 1 out of 4 in 2zjy

Go back to Fluorine Binding Sites List in 2zjy
Fluorine binding site 1 out of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F356

b:8.6
occ:1.00
F1 A:ALF356 0.0 8.6 1.0
AL A:ALF356 1.8 2.2 1.0
F2 A:ALF356 2.1 2.2 1.0
NH2 A:ARG178 2.7 12.7 1.0
F4 A:ALF356 2.8 30.2 1.0
NE2 A:GLN204 2.8 22.9 1.0
O3B A:GDP355 2.8 22.1 1.0
N A:GLU43 3.1 8.0 1.0
F3 A:ALF356 3.5 25.1 1.0
CD A:GLN204 3.7 21.8 1.0
CZ A:ARG178 3.7 15.2 1.0
OE1 A:GLN204 3.7 15.4 1.0
CA A:GLU43 3.9 6.8 1.0
NH1 A:ARG178 3.9 18.9 1.0
CA A:GLY42 4.0 12.9 1.0
C A:GLY42 4.0 11.3 1.0
PB A:GDP355 4.0 18.1 1.0
CG A:GLU43 4.1 5.5 1.0
O1B A:GDP355 4.1 19.2 1.0
O A:HOH533 4.4 2.2 1.0
CB A:GLU43 4.5 8.2 1.0
N A:THR181 4.6 16.8 1.0
CG A:LYS180 4.7 24.6 1.0
CA A:LYS180 4.8 11.8 1.0
N A:GLY203 4.9 8.3 1.0
NE A:ARG178 4.9 17.1 1.0
O A:HOH424 5.0 11.9 1.0
MG A:MG357 5.0 2.6 1.0

Fluorine binding site 2 out of 4 in 2zjy

Go back to Fluorine Binding Sites List in 2zjy
Fluorine binding site 2 out of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F356

b:2.2
occ:1.00
F2 A:ALF356 0.0 2.2 1.0
AL A:ALF356 1.8 2.2 1.0
F1 A:ALF356 2.1 8.6 1.0
F3 A:ALF356 2.5 25.1 1.0
N A:THR181 2.8 16.8 1.0
O3B A:GDP355 3.0 22.1 1.0
NE2 A:GLN204 3.1 22.9 1.0
NH2 A:ARG178 3.2 12.7 1.0
O A:HOH533 3.2 2.2 1.0
F4 A:ALF356 3.4 30.2 1.0
CB A:THR181 3.4 12.9 1.0
CA A:THR181 3.5 18.6 1.0
O A:THR181 3.5 25.8 1.0
MG A:MG357 3.6 2.6 1.0
C A:LYS180 3.7 11.5 1.0
CA A:LYS180 3.8 11.8 1.0
OG1 A:THR181 3.9 18.5 1.0
CG A:LYS180 3.9 24.6 1.0
C A:THR181 4.0 21.7 1.0
PB A:GDP355 4.2 18.1 1.0
CD A:GLN204 4.4 21.8 1.0
CZ A:ARG178 4.4 15.2 1.0
CB A:LYS180 4.4 13.3 1.0
CA A:GLY202 4.6 10.4 1.0
O2B A:GDP355 4.6 28.6 1.0
N A:GLY203 4.6 8.3 1.0
O A:VAL179 4.6 5.8 1.0
O1B A:GDP355 4.7 19.2 1.0
CG2 A:THR181 4.7 11.7 1.0
O A:LYS180 4.9 10.5 1.0
OE1 A:GLN204 4.9 15.4 1.0
N A:LYS180 5.0 11.9 1.0
N A:GLU43 5.0 8.0 1.0

Fluorine binding site 3 out of 4 in 2zjy

Go back to Fluorine Binding Sites List in 2zjy
Fluorine binding site 3 out of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F356

b:25.1
occ:1.00
F3 A:ALF356 0.0 25.1 1.0
AL A:ALF356 1.8 2.2 1.0
F2 A:ALF356 2.5 2.2 1.0
F4 A:ALF356 2.6 30.2 1.0
MG A:MG357 2.7 2.6 1.0
O A:VAL201 2.9 6.8 1.0
CA A:GLY202 3.1 10.4 1.0
N A:GLY203 3.1 8.3 1.0
CE A:LYS46 3.2 16.7 1.0
NZ A:LYS46 3.3 4.1 1.0
C A:GLY202 3.4 7.8 1.0
F1 A:ALF356 3.5 8.6 1.0
CB A:THR181 3.5 12.9 1.0
O3B A:GDP355 3.5 22.1 1.0
C A:VAL201 3.7 7.0 1.0
O2B A:GDP355 3.7 28.6 1.0
PB A:GDP355 3.8 18.1 1.0
N A:GLY202 3.8 7.8 1.0
O A:THR181 3.9 25.8 1.0
O1B A:GDP355 4.0 19.2 1.0
O A:HOH533 4.0 2.2 1.0
NE2 A:GLN204 4.1 22.9 1.0
OG1 A:THR181 4.2 18.5 1.0
CG2 A:THR181 4.2 11.7 1.0
CA A:GLY203 4.2 11.0 1.0
CA A:THR181 4.4 18.6 1.0
N A:THR181 4.4 16.8 1.0
O A:GLY202 4.5 14.0 1.0
C A:THR181 4.5 21.7 1.0
CD A:LYS46 4.7 8.0 1.0
OD1 A:ASP200 4.8 24.2 1.0

Fluorine binding site 4 out of 4 in 2zjy

Go back to Fluorine Binding Sites List in 2zjy
Fluorine binding site 4 out of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F356

b:30.2
occ:1.00
F4 A:ALF356 0.0 30.2 1.0
AL A:ALF356 1.7 2.2 1.0
F3 A:ALF356 2.6 25.1 1.0
N A:GLY203 2.6 8.3 1.0
F1 A:ALF356 2.8 8.6 1.0
CA A:GLY203 2.9 11.0 1.0
NZ A:LYS46 2.9 4.1 1.0
CA A:GLY42 3.1 12.9 1.0
NE2 A:GLN204 3.2 22.9 1.0
O A:ALA41 3.3 12.8 1.0
F2 A:ALF356 3.4 2.2 1.0
N A:GLU43 3.5 8.0 1.0
C A:GLY203 3.6 15.5 1.0
N A:GLY42 3.6 12.8 1.0
C A:ALA41 3.7 10.0 1.0
C A:GLY202 3.7 7.8 1.0
O1B A:GDP355 3.8 19.2 1.0
N A:GLN204 3.8 16.1 1.0
C A:GLY42 3.8 11.3 1.0
CE A:LYS46 3.9 16.7 1.0
CD A:GLN204 4.0 21.8 1.0
O3B A:GDP355 4.0 22.1 1.0
CA A:GLY202 4.2 10.4 1.0
O A:GLY40 4.3 7.0 1.0
OE1 A:GLN204 4.4 15.4 1.0
PB A:GDP355 4.4 18.1 1.0
O A:GLY203 4.5 15.3 1.0
O A:GLY202 4.7 14.0 1.0
CA A:GLU43 4.8 6.8 1.0
O A:VAL201 4.8 6.8 1.0
CA A:ALA41 5.0 10.4 1.0
MG A:MG357 5.0 2.6 1.0
CG A:GLN204 5.0 15.0 1.0
O A:GLY42 5.0 11.7 1.0

Reference:

T.Morikawa, A.Muroya, S.Sugio, K.Wakamatsu, T.Kohno. How Gpcrs Activate G Proteins: Structural Changes Form C-Terminal Tail to Gdp Binding Pocket To Be Published.
Page generated: Mon Jul 14 15:06:37 2025

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