Fluorine in PDB 6og8: Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)

The structure of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51), PDB code: 6og8 was solved by C.-Y.Lin, A.Deng, S.G.Boxer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.28 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.073, 70.072, 61.072, 90.00, 95.93, 90.00
R / Rfree (%)	15.9 / 19

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 25;

Binding sites:

The binding sites of Fluorine atom in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) (pdb code 6og8). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 25 binding sites of Fluorine where determined in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51), PDB code: 6og8:
Jump to Fluorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Fluorine binding site 1 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F15 b:13.0 occ:0.59 F B:MFC15 0.0 13.0 0.6 CE2 B:MFC15 1.4 8.8 0.6 CE1 B:MFC15 1.4 8.6 0.4 CZ B:MFC15 2.3 7.5 0.6 CZ B:MFC15 2.4 8.4 0.4 CD2 B:MFC15 2.4 8.2 0.6 CD1 B:MFC15 2.4 7.9 0.4 OH B:MFC15 2.7 7.8 0.6 OH B:MFC15 2.8 8.3 0.4 OG1 B:THR153 2.9 8.0 0.2 OE2 B:GLU172 3.3 18.4 1.0 OG1 B:THR155 3.3 7.9 1.0 CE2 B:PHE95 3.4 13.6 1.0 O B:HOH435 3.5 10.9 1.0 CD B:GLU172 3.6 14.4 1.0 CE1 B:MFC15 3.6 7.6 0.6 CE2 B:MFC15 3.6 7.7 0.4 CG2 B:THR153 3.6 9.6 0.2 CG2 B:MFC15 3.6 6.7 0.6 CG2 B:MFC15 3.6 6.8 0.4 CB B:THR153 3.7 8.7 0.8 OG1 B:THR153 3.7 10.8 0.8 CB B:THR153 3.7 8.9 0.2 CG2 B:THR153 4.0 8.0 0.8 OE1 B:GLU172 4.0 17.1 1.0 CD1 B:MFC15 4.1 7.7 0.6 CD2 B:MFC15 4.1 7.6 0.4 CD2 B:PHE95 4.1 12.0 1.0 CZ B:PHE95 4.2 11.2 1.0 CG B:GLU172 4.2 12.5 1.0 CG1 B:VAL11 4.3 5.9 1.0 CB B:THR155 4.6 9.1 1.0 O B:HOH363 4.7 21.2 1.0 F B:MFC15 4.7 9.7 0.4 OG1 B:MFC15 4.9 8.8 0.6 OD2 B:ASP98 4.9 17.5 1.0 CB2 B:MFC15 5.0 6.7 0.4 CB2 B:MFC15 5.0 6.5 0.6 OG1 B:MFC15 5.0 8.9 0.4

Fluorine binding site 2 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F15 b:9.7 occ:0.41 F B:MFC15 0.0 9.7 0.4 CE2 B:MFC15 1.4 7.7 0.4 CE1 B:MFC15 1.4 7.6 0.6 CZ B:MFC15 2.4 8.4 0.4 CZ B:MFC15 2.4 7.5 0.6 CD1 B:MFC15 2.4 7.7 0.6 CD2 B:MFC15 2.4 7.6 0.4 OH B:MFC15 2.7 8.3 0.4 OH B:MFC15 2.8 7.8 0.6 OD2 B:ASP98 3.0 17.5 1.0 CG2 B:VAL100 3.0 15.4 1.0 CE2 B:PHE115 3.2 8.9 1.0 CG B:ASP98 3.4 15.3 1.0 CB B:ASP98 3.4 14.1 1.0 CD2 B:PHE115 3.6 10.8 1.0 CE2 B:MFC15 3.6 8.8 0.6 CE1 B:MFC15 3.6 8.6 0.4 CG2 B:MFC15 3.7 6.7 0.6 CG2 B:MFC15 3.7 6.8 0.4 CZ B:PHE115 3.9 8.1 1.0 CD2 B:MFC15 4.1 8.2 0.6 CD1 B:MFC15 4.1 7.9 0.4 CG2 B:THR12 4.3 7.9 1.0 OD1 B:ASP98 4.4 15.8 1.0 CG2 B:VAL117 4.5 11.1 1.0 CG2 B:THR153 4.5 9.6 0.2 CB B:VAL100 4.5 13.5 1.0 CG B:PHE115 4.6 12.3 1.0 F B:MFC15 4.7 13.0 0.6 CG2 B:THR153 4.7 8.0 0.8 OG1 B:THR153 4.8 10.8 0.8 CE1 B:PHE115 4.8 10.4 1.0 CB2 B:MFC15 4.9 6.5 0.6 CA B:ASP98 4.9 10.5 1.0 CB2 B:MFC15 4.9 6.7 0.4 O B:HOH507 5.0 15.7 1.0

Fluorine binding site 3 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F24 b:13.3 occ:1.00 F B:YOF24 0.0 13.3 1.0 CE1 B:YOF24 1.3 11.0 1.0 CZ B:YOF24 2.4 11.1 1.0 CD1 B:YOF24 2.4 8.4 1.0 OH B:YOF24 2.7 8.9 1.0 O B:HOH368 3.0 10.2 1.0 CB B:SER22 3.4 8.3 1.0 CD2 B:PHE34 3.4 7.9 1.0 O B:GLN19 3.6 7.4 1.0 CE2 B:YOF24 3.6 10.9 1.0 CG B:YOF24 3.6 8.8 1.0 CD2 B:LEU151 3.7 9.4 1.0 CE2 B:PHE34 3.7 11.5 1.0 OG B:SER22 3.8 10.3 1.0 OD1 B:ASP32 3.9 10.5 1.0 CD2 B:YOF24 4.1 11.4 1.0 O B:SER22 4.4 8.5 1.0 CG B:ASP32 4.4 11.5 1.0 CA B:SER22 4.5 7.3 1.0 CD1 B:LEU151 4.5 8.2 1.0 CG B:PHE34 4.6 7.8 1.0 C B:SER22 4.6 8.6 1.0 OD2 B:ASP32 4.7 12.1 1.0 C B:GLN19 4.7 8.5 1.0 CG B:LEU151 4.7 8.2 1.0 CB B:ALA176 4.8 10.9 1.0 N B:SER22 4.9 7.3 1.0 O B:HOH377 4.9 11.1 1.0 NZ B:LYS35 4.9 8.7 1.0 CB B:YOF24 4.9 9.4 1.0

Fluorine binding site 4 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F42 b:12.7 occ:0.59 F B:YOF42 0.0 12.7 0.6 CE2 B:YOF42 1.3 10.3 0.4 CE1 B:YOF42 1.4 9.8 0.6 CZ B:YOF42 2.1 10.3 0.4 CZ B:YOF42 2.3 10.1 0.6 OH B:YOF42 2.4 9.7 0.4 CD1 B:YOF42 2.4 9.8 0.6 CD2 B:YOF42 2.5 10.0 0.4 OH B:YOF42 2.7 9.3 0.6 O B:PHE34 3.0 9.1 1.0 CE B:MET38 3.1 11.1 1.0 CB B:PHE34 3.2 8.2 1.0 CE1 B:YOF42 3.4 9.9 0.4 CB B:CYS20 3.4 9.8 1.0 CA B:PHE34 3.4 8.3 1.0 C B:PHE34 3.5 11.8 1.0 CE2 B:YOF42 3.6 9.9 0.6 CG B:YOF42 3.7 9.1 0.4 CG B:YOF42 3.7 9.1 0.6 SG B:CYS20 3.8 9.0 1.0 CD1 B:PHE34 4.0 9.0 1.0 CD1 B:YOF42 4.0 9.1 0.4 CG B:PHE34 4.1 7.8 1.0 CD2 B:YOF42 4.1 8.9 0.6 N B:CYS20 4.1 9.1 1.0 CA B:CYS20 4.2 9.3 1.0 CG1 B:VAL62 4.4 10.3 1.0 F B:YOF42 4.4 10.3 0.4 N B:LYS35 4.6 10.2 1.0 SD B:MET38 4.8 10.7 1.0 CG B:MET38 4.9 8.8 1.0 CB B:VAL62 4.9 8.8 1.0 N B:PHE34 4.9 8.9 1.0 CB B:YOF42 5.0 9.0 0.6

Fluorine binding site 5 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 5 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F42 b:10.3 occ:0.41 F B:YOF42 0.0 10.3 0.4 CE2 B:YOF42 1.2 9.9 0.6 CE1 B:YOF42 1.3 9.9 0.4 CZ B:YOF42 2.1 10.1 0.6 CZ B:YOF42 2.3 10.3 0.4 CD2 B:YOF42 2.3 8.9 0.6 CD1 B:YOF42 2.4 9.1 0.4 OH B:YOF42 2.5 9.3 0.6 OH B:YOF42 2.7 9.7 0.4 OE1 B:GLN44 2.8 12.2 1.0 O B:HOH331 2.9 14.5 1.0 ND2 B:ASN135 3.2 12.4 1.0 CE1 B:YOF42 3.4 9.8 0.6 CG2 B:VAL62 3.4 9.0 1.0 CB B:GLN44 3.5 8.2 1.0 CG B:YOF42 3.5 9.1 0.6 CD B:GLN44 3.6 10.2 1.0 CE2 B:YOF42 3.6 10.3 0.4 CG B:YOF42 3.6 9.1 0.4 CB B:VAL62 3.9 8.8 1.0 CD1 B:YOF42 3.9 9.8 0.6 O B:MFC15 4.0 7.2 0.6 O B:MFC15 4.0 7.3 0.4 CG B:GLN44 4.1 8.9 1.0 CD2 B:YOF42 4.1 10.0 0.4 O B:HOH365 4.2 7.3 1.0 CG1 B:VAL62 4.4 10.3 1.0 F B:YOF42 4.4 12.7 0.6 CG B:ASN135 4.5 13.0 1.0 NE2 B:GLN44 4.5 9.5 1.0 CA B:GLN44 4.6 9.4 1.0 N B:GLN44 4.7 9.8 1.0 O B:YOF42 4.8 10.2 0.4 CD1 B:PHE34 4.8 9.0 1.0 O B:YOF42 4.8 10.2 0.6 C B:MFC15 4.9 7.5 0.6 C B:MFC15 4.9 7.6 0.4 CB B:YOF42 4.9 9.0 0.6 CB B:YOF42 5.0 9.2 0.4

Fluorine binding site 6 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 6 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F56 b:13.4 occ:1.00 F B:YOF56 0.0 13.4 1.0 CE1 B:YOF56 1.4 8.8 1.0 CZ B:YOF56 2.4 7.0 1.0 CD1 B:YOF56 2.4 7.3 1.0 OH B:YOF56 2.7 7.8 1.0 CZ B:PHE80 3.1 11.8 1.0 CG2 B:VAL215 3.2 7.7 1.0 CE2 B:PHE80 3.2 12.3 1.0 CG1 B:VAL215 3.3 9.4 1.0 CG B:YOF56 3.6 7.1 1.0 CE2 B:YOF56 3.6 6.9 1.0 CG1 B:VAL5 3.7 10.3 0.9 CE1 B:PHE80 3.9 11.2 1.0 CG2 B:VAL5 3.9 9.2 0.1 CB B:VAL215 3.9 8.0 1.0 CD2 B:PHE80 4.0 9.5 1.0 CD2 B:YOF56 4.1 6.9 1.0 CG1 B:VAL5 4.2 8.5 0.1 CG2 B:ILE86 4.3 7.9 0.2 CG2 B:ILE86 4.3 8.0 0.8 CD1 B:PHE80 4.5 11.8 1.0 CA B:GLY77 4.5 8.6 1.0 CG B:PHE80 4.6 8.4 1.0 CB B:VAL5 4.6 8.1 0.9 CB B:VAL5 4.6 8.8 0.1 CD2 B:LEU75 4.8 7.3 1.0 CE2 B:PHE50 4.8 8.6 1.0 CB B:YOF56 5.0 7.2 1.0 CG2 B:VAL5 5.0 8.1 0.9

Fluorine binding site 7 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 7 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F93 b:11.1 occ:0.48 F B:YOF93 0.0 11.1 0.5 CE2 B:YOF93 1.3 9.6 0.5 CE1 B:YOF93 1.3 9.6 0.5 CZ B:YOF93 2.2 9.4 0.5 CD2 B:YOF93 2.3 8.9 0.5 CD1 B:YOF93 2.4 9.3 0.5 CZ B:YOF93 2.4 9.2 0.5 OH B:YOF93 2.6 8.9 0.5 OH B:YOF93 2.7 9.0 0.5 CB B:TRP7 3.1 8.3 1.0 NZ B:LYS159 3.4 10.6 1.0 CG B:TRP7 3.4 9.3 1.0 CE1 B:YOF93 3.5 8.4 0.5 CD1 B:TRP7 3.5 6.9 1.0 CG B:YOF93 3.5 8.3 0.5 O B:HOH477 3.6 15.5 1.0 CE2 B:YOF93 3.6 8.5 0.5 CG B:YOF93 3.6 8.2 0.5 CB B:MET168 3.9 8.1 1.0 CG B:MET168 3.9 8.6 1.0 CD1 B:YOF93 4.0 7.9 0.5 CE B:LYS159 4.0 8.1 1.0 O B:HOH531 4.0 5.9 1.0 O B:HIS167 4.1 8.8 1.0 CD2 B:YOF93 4.1 8.0 0.5 SD B:MET168 4.2 8.9 1.0 CA B:MET168 4.4 6.7 1.0 CA B:TRP7 4.5 6.5 1.0 CD2 B:TRP7 4.5 8.1 1.0 CD B:PRO8 4.6 7.0 1.0 F B:YOF93 4.6 10.1 0.5 NE1 B:TRP7 4.6 8.3 1.0 CD1 B:LEU157 4.8 7.2 1.0 CD B:LYS159 4.8 7.7 1.0 CB B:YOF93 4.9 8.1 0.5 CB B:YOF93 4.9 8.2 0.5 N B:TRP7 4.9 6.8 1.0 C B:HIS167 5.0 8.2 1.0

Fluorine binding site 8 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 8 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F93 b:10.1 occ:0.52 F B:YOF93 0.0 10.1 0.5 CE2 B:YOF93 1.2 8.5 0.5 CE1 B:YOF93 1.3 8.4 0.5 CZ B:YOF93 2.2 9.2 0.5 CD2 B:YOF93 2.3 8.0 0.5 CD1 B:YOF93 2.3 7.9 0.5 CZ B:YOF93 2.4 9.4 0.5 OH B:YOF93 2.7 9.0 0.5 OH B:YOF93 2.8 8.9 0.5 C B:SER158 2.8 6.7 1.0 CA B:SER158 2.8 7.1 1.0 N B:SER158 3.0 6.6 1.0 O B:SER158 3.1 6.7 1.0 C B:LEU157 3.3 7.6 1.0 O B:LEU157 3.4 9.3 1.0 N B:LYS159 3.4 10.5 1.0 CE1 B:YOF93 3.5 9.6 0.5 CG B:LYS159 3.5 7.5 1.0 CG B:YOF93 3.5 8.2 0.5 CG B:YOF93 3.6 8.3 0.5 CE2 B:YOF93 3.6 9.6 0.5 CD B:LYS159 3.9 7.7 1.0 CD1 B:YOF93 4.0 9.3 0.5 O B:HOH517 4.0 18.1 1.0 CE B:LYS159 4.0 8.1 1.0 CD2 B:YOF93 4.1 8.9 0.5 O B:HOH442 4.1 14.2 1.0 CB B:LEU157 4.1 6.9 1.0 CA B:LYS159 4.3 7.1 1.0 CB B:SER158 4.3 7.5 1.0 O B:HOH648 4.3 35.9 1.0 CA B:LEU157 4.3 6.9 1.0 CB B:LYS159 4.4 7.3 1.0 F B:YOF93 4.6 11.1 0.5 O A:HOH607 4.7 28.0 1.0 CB B:YOF93 4.8 8.2 0.5 O A:HOH594 4.8 30.9 1.0 CB B:YOF93 4.9 8.1 0.5 NZ B:LYS159 5.0 10.6 1.0

Fluorine binding site 9 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 9 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F101 b:24.3 occ:0.48 F B:YOF101 0.0 24.3 0.5 CE2 B:YOF101 1.3 24.5 0.5 CE1 B:YOF101 1.3 24.6 0.5 CZ B:YOF101 2.3 27.0 0.5 CD2 B:YOF101 2.3 22.1 0.5 CD1 B:YOF101 2.4 22.1 0.5 CZ B:YOF101 2.4 27.0 0.5 OH B:YOF101 2.7 30.0 0.5 OH B:YOF101 2.8 30.0 0.5 CE1 B:YOF101 3.5 25.7 0.5 CG B:YOF101 3.6 20.4 0.5 O B:ASN99 3.6 18.4 1.0 CG B:YOF101 3.6 20.4 0.5 CB B:ASN99 3.6 16.2 1.0 CE2 B:YOF101 3.6 25.8 0.5 O B:HOH350 3.7 26.4 1.0 CD1 B:YOF101 4.0 23.6 0.5 CD2 B:YOF101 4.1 23.6 0.5 C B:ASN99 4.3 16.0 1.0 CA B:ASN99 4.6 16.0 1.0 F B:YOF101 4.6 27.0 0.5 CG B:ASN99 4.7 19.1 1.0 ND2 B:ASN99 4.7 23.0 1.0 O B:HOH410 4.8 14.0 1.0 CB B:YOF101 4.9 15.8 0.5 CB B:YOF101 4.9 15.8 0.5

Fluorine binding site 10 out of 25 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 10 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:F101 b:27.0 occ:0.52 F B:YOF101 0.0 27.0 0.5 CE2 B:YOF101 1.3 25.8 0.5 CE1 B:YOF101 1.3 25.7 0.5 CZ B:YOF101 2.2 27.0 0.5 CZ B:YOF101 2.3 27.0 0.5 CD1 B:YOF101 2.4 23.6 0.5 CD2 B:YOF101 2.4 23.6 0.5 OH B:YOF101 2.6 30.0 0.5 OH B:YOF101 2.7 30.0 0.5 CD1 B:YOF150 3.3 26.2 1.0 O B:HOH570 3.3 32.0 1.0 CE1 B:YOF101 3.5 24.6 0.5 CE2 B:YOF101 3.6 24.5 0.5 CG B:YOF101 3.6 20.4 0.5 CG B:YOF101 3.6 20.4 0.5 CE1 B:YOF150 3.9 31.2 1.0 F B:YOF150 4.0 38.2 1.0 CD1 B:YOF101 4.1 22.1 0.5 CG B:YOF150 4.1 20.8 1.0 CD2 B:YOF101 4.1 22.1 0.5 CB B:YOF150 4.3 17.0 1.0 F B:YOF101 4.6 24.3 0.5 O B:HOH544 5.0 31.0 1.0 CB B:YOF101 5.0 15.8 0.5 CB B:YOF101 5.0 15.8 0.5

C.-Y.Lin, A.Deng, S.G.Boxer. Crystal Structure of Green Fluorescent Protein (Gfp); S65T, H148D with Globally Incorporated 3-F1Y; Circular Permutant (50-51) To Be Published.