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Fluorine in PDB 8uh7: Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog

Protein crystallography data

The structure of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog, PDB code: 8uh7 was solved by C.L.Gee, K.Marcus, B.A.Kelch, D.L.Makino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.65 / 2.63
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 93.351, 118.325, 132.882, 90, 102.05, 90
R / Rfree (%) 19.3 / 24.5

Other elements in 8uh7:

The structure of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog also contains other interesting chemical elements:

Magnesium (Mg) 4 atoms

Fluorine Binding Sites:

The binding sites of Fluorine atom in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog (pdb code 8uh7). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 9 binding sites of Fluorine where determined in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog, PDB code: 8uh7:
Jump to Fluorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Fluorine binding site 1 out of 9 in 8uh7

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Fluorine binding site 1 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
B:F401

b:49.5
occ:1.00
F1 B:08T401 0.0 49.5 1.0
BE B:08T401 1.5 39.5 1.0
MG B:MG402 2.0 22.4 1.0
O2B B:08T401 2.3 24.9 1.0
F2 B:08T401 2.4 28.4 1.0
O3B B:08T401 2.5 35.3 1.0
F3 B:08T401 2.5 37.3 1.0
OE2 B:GLU108 2.7 44.3 1.0
PB B:08T401 2.9 28.8 1.0
NH2 C:ARG151 3.5 40.5 1.0
CD B:GLU108 3.6 50.6 1.0
OG1 B:THR57 3.8 33.1 1.0
O1B B:08T401 3.9 21.8 1.0
NH2 B:ARG205 4.0 38.5 1.0
CE B:LYS56 4.2 21.6 1.0
OE1 B:GLU108 4.2 34.3 1.0
O3A B:08T401 4.2 19.0 1.0
O1A B:08T401 4.4 27.5 1.0
NZ B:LYS56 4.5 16.0 1.0
CZ C:ARG151 4.5 25.9 1.0
NH1 C:ARG151 4.6 24.9 1.0
CG B:GLU108 4.6 43.0 1.0
ND2 B:ASN139 4.6 42.4 1.0
NH1 B:ARG205 4.9 28.1 1.0
PA B:08T401 4.9 25.9 1.0
CZ B:ARG205 5.0 28.4 1.0
N B:GLY53 5.0 32.4 1.0

Fluorine binding site 2 out of 9 in 8uh7

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Fluorine binding site 2 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
B:F401

b:28.4
occ:1.00
F2 B:08T401 0.0 28.4 1.0
BE B:08T401 1.5 39.5 1.0
F3 B:08T401 2.4 37.3 1.0
F1 B:08T401 2.4 49.5 1.0
O3B B:08T401 2.5 35.3 1.0
NH1 C:ARG151 2.6 24.9 1.0
NH2 C:ARG151 2.8 40.5 1.0
CZ C:ARG151 3.1 25.9 1.0
NH1 B:ARG205 3.1 28.1 1.0
NH2 B:ARG205 3.3 38.5 1.0
CZ B:ARG205 3.6 28.4 1.0
PB B:08T401 3.9 28.8 1.0
N B:GLY53 3.9 32.4 1.0
CB B:PRO52 4.0 22.2 1.0
MG B:MG402 4.0 22.4 1.0
O2B B:08T401 4.0 24.9 1.0
CB C:PRO147 4.2 16.7 1.0
CA B:PRO52 4.3 21.8 1.0
NE C:ARG151 4.4 16.7 1.0
OE2 B:GLU108 4.6 44.3 1.0
C B:PRO52 4.6 24.5 1.0
CA C:PRO147 4.7 17.7 1.0
O3A B:08T401 4.7 19.0 1.0
ND2 B:ASN139 4.8 42.4 1.0
CA B:GLY53 4.8 27.7 1.0
NE B:ARG205 4.8 22.5 1.0
OD1 B:ASN139 4.9 30.9 1.0
CG C:PRO147 5.0 16.3 1.0
O1B B:08T401 5.0 21.8 1.0

Fluorine binding site 3 out of 9 in 8uh7

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Fluorine binding site 3 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
B:F401

b:37.3
occ:1.00
F3 B:08T401 0.0 37.3 1.0
BE B:08T401 1.5 39.5 1.0
F2 B:08T401 2.4 28.4 1.0
F1 B:08T401 2.5 49.5 1.0
O3B B:08T401 2.6 35.3 1.0
ND2 B:ASN139 2.7 42.4 1.0
NZ B:LYS56 3.2 16.0 1.0
CE B:LYS56 3.5 21.6 1.0
CA B:PRO52 3.5 21.8 1.0
CG B:ASN139 3.6 30.7 1.0
PB B:08T401 3.8 28.8 1.0
OD1 B:ASN139 3.8 30.9 1.0
N B:GLY53 3.8 32.4 1.0
CB B:PRO52 3.9 22.2 1.0
OE2 B:GLU108 4.0 44.3 1.0
O2B B:08T401 4.1 24.9 1.0
O1B B:08T401 4.1 21.8 1.0
C B:PRO52 4.2 24.5 1.0
OE1 B:GLU108 4.4 34.3 1.0
MG B:MG402 4.5 22.4 1.0
CD B:GLU108 4.5 50.6 1.0
N B:PRO52 4.5 23.3 1.0
O B:SER51 4.6 30.4 1.0
NH1 C:ARG151 4.8 24.9 1.0
NH2 C:ARG151 4.8 40.5 1.0
O B:SER49 4.9 39.6 1.0
NH1 B:ARG205 4.9 28.1 1.0
C B:SER51 4.9 23.8 1.0
CB C:PRO147 5.0 16.7 1.0
CD B:LYS56 5.0 26.8 1.0
CG C:PRO147 5.0 16.3 1.0

Fluorine binding site 4 out of 9 in 8uh7

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Fluorine binding site 4 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
C:F401

b:15.3
occ:1.00
F1 C:08T401 0.0 15.3 1.0
BE C:08T401 1.5 18.0 1.0
MG C:MG402 2.1 12.7 1.0
O2B C:08T401 2.2 17.9 1.0
F2 C:08T401 2.4 12.3 1.0
O3B C:08T401 2.5 10.2 1.0
F3 C:08T401 2.5 18.0 1.0
OE2 C:GLU108 2.6 40.8 1.0
PB C:08T401 2.9 16.2 1.0
NH2 D:ARG151 3.4 20.6 1.0
CD C:GLU108 3.7 44.1 1.0
NH2 C:ARG205 3.9 18.6 1.0
O1B C:08T401 3.9 18.9 1.0
OG1 C:THR57 3.9 26.3 1.0
O3A C:08T401 4.1 15.5 1.0
CE C:LYS56 4.1 17.0 1.0
OE1 C:GLU108 4.1 31.9 1.0
NZ C:LYS56 4.2 9.1 1.0
NH1 D:ARG151 4.3 12.1 1.0
CZ D:ARG151 4.3 12.4 1.0
CG2 C:THR137 4.5 19.1 1.0
O1A C:08T401 4.5 15.3 1.0
OD1 C:ASN139 4.5 33.6 1.0
CG C:GLU108 4.9 27.5 1.0
PA C:08T401 4.9 22.0 1.0
CZ C:ARG205 4.9 13.5 1.0
N C:GLY53 5.0 18.8 1.0
CB C:THR57 5.0 17.2 1.0

Fluorine binding site 5 out of 9 in 8uh7

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Fluorine binding site 5 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 5 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
C:F401

b:12.3
occ:1.00
F2 C:08T401 0.0 12.3 1.0
BE C:08T401 1.5 18.0 1.0
F3 C:08T401 2.4 18.0 1.0
F1 C:08T401 2.4 15.3 1.0
O3B C:08T401 2.5 10.2 1.0
NH1 D:ARG151 2.7 12.1 1.0
NH2 C:ARG205 2.9 18.6 1.0
NH1 C:ARG205 3.1 12.3 1.0
NH2 D:ARG151 3.2 20.6 1.0
CZ D:ARG151 3.3 12.4 1.0
CZ C:ARG205 3.4 13.5 1.0
CB C:PRO52 3.7 16.7 1.0
PB C:08T401 3.7 16.2 1.0
N C:GLY53 3.8 18.8 1.0
O2B C:08T401 3.8 17.9 1.0
CA C:PRO52 4.0 12.5 1.0
MG C:MG402 4.1 12.7 1.0
CB D:PRO147 4.3 17.3 1.0
O3A C:08T401 4.4 15.5 1.0
C C:PRO52 4.4 12.7 1.0
OE2 C:GLU108 4.4 40.8 1.0
NE D:ARG151 4.5 12.4 1.0
NE C:ARG205 4.7 9.1 1.0
ND2 C:ASN139 4.7 17.1 1.0
CA C:GLY53 4.7 17.1 1.0
CA D:PRO147 4.7 17.0 1.0
OD1 C:ASN139 4.7 33.6 1.0
NZ C:LYS56 4.9 9.1 1.0
CG C:PRO52 4.9 7.3 1.0
CG D:PRO147 5.0 7.4 1.0
O1B C:08T401 5.0 18.9 1.0

Fluorine binding site 6 out of 9 in 8uh7

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Fluorine binding site 6 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 6 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
C:F401

b:18.0
occ:1.00
F3 C:08T401 0.0 18.0 1.0
BE C:08T401 1.5 18.0 1.0
F2 C:08T401 2.4 12.3 1.0
F1 C:08T401 2.5 15.3 1.0
O3B C:08T401 2.6 10.2 1.0
ND2 C:ASN139 2.8 17.1 1.0
NZ C:LYS56 3.2 9.1 1.0
OD1 C:ASN139 3.2 33.6 1.0
CG C:ASN139 3.3 14.1 1.0
CE C:LYS56 3.4 17.0 1.0
CA C:PRO52 3.5 12.5 1.0
CB C:PRO52 3.9 16.7 1.0
PB C:08T401 3.9 16.2 1.0
N C:GLY53 4.0 18.8 1.0
O2B C:08T401 4.1 17.9 1.0
OE2 C:GLU108 4.2 40.8 1.0
C C:PRO52 4.3 12.7 1.0
O C:SER51 4.3 26.3 1.0
N C:PRO52 4.5 10.6 1.0
O1B C:08T401 4.5 18.9 1.0
OE1 C:GLU108 4.5 31.9 1.0
MG C:MG402 4.5 12.7 1.0
NH1 D:ARG151 4.7 12.1 1.0
CD C:GLU108 4.7 44.1 1.0
CB C:ASN139 4.7 9.7 1.0
CG C:PRO52 4.8 7.3 1.0
C C:SER51 4.8 14.4 1.0
CG D:PRO147 4.9 7.4 1.0
CD C:LYS56 4.9 14.8 1.0
CB D:PRO147 4.9 17.3 1.0
NH2 D:ARG151 4.9 20.6 1.0
NH2 C:ARG205 5.0 18.6 1.0

Fluorine binding site 7 out of 9 in 8uh7

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Fluorine binding site 7 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 7 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
D:F401

b:21.4
occ:1.00
F1 D:08T401 0.0 21.4 1.0
BE D:08T401 1.5 21.5 1.0
MG D:MG402 2.0 11.3 1.0
O2B D:08T401 2.2 25.2 1.0
F2 D:08T401 2.4 18.7 1.0
O3B D:08T401 2.5 16.5 1.0
F3 D:08T401 2.5 18.3 1.0
OE2 D:GLU108 2.7 53.2 1.0
NH2 E:ARG151 2.8 25.2 1.0
PB D:08T401 2.9 24.9 1.0
O D:HOH508 3.2 23.4 1.0
NH2 D:ARG205 3.5 27.7 1.0
CD D:GLU108 3.7 62.7 1.0
CZ E:ARG151 3.8 24.3 1.0
O1B D:08T401 4.0 30.8 1.0
OG1 D:THR57 4.0 22.9 1.0
NH1 E:ARG151 4.0 14.8 1.0
OE1 D:GLU108 4.0 54.5 1.0
O3A D:08T401 4.1 15.2 1.0
NZ D:LYS56 4.2 19.3 1.0
O1A D:08T401 4.4 19.2 1.0
CZ D:ARG205 4.5 28.1 1.0
NH1 D:ARG205 4.7 22.0 1.0
OE1 E:GLU126 4.8 23.6 1.0
OD1 D:ASN139 4.8 27.1 1.0
PA D:08T401 4.9 14.7 1.0
N D:GLY53 4.9 18.5 1.0
CG D:GLU108 5.0 35.2 1.0

Fluorine binding site 8 out of 9 in 8uh7

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Fluorine binding site 8 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 8 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
D:F401

b:18.7
occ:1.00
F2 D:08T401 0.0 18.7 1.0
BE D:08T401 1.5 21.5 1.0
F3 D:08T401 2.4 18.3 1.0
F1 D:08T401 2.4 21.4 1.0
O3B D:08T401 2.5 16.5 1.0
NH1 E:ARG151 2.5 14.8 1.0
NH1 D:ARG205 3.0 22.0 1.0
NH2 E:ARG151 3.0 25.2 1.0
NH2 D:ARG205 3.1 27.7 1.0
CZ E:ARG151 3.2 24.3 1.0
CB D:PRO52 3.3 16.9 1.0
CZ D:ARG205 3.4 28.1 1.0
N D:GLY53 3.6 18.5 1.0
CA D:PRO52 3.8 14.6 1.0
PB D:08T401 3.8 24.9 1.0
O2B D:08T401 4.0 25.2 1.0
MG D:MG402 4.2 11.3 1.0
C D:PRO52 4.2 19.1 1.0
CB E:PRO147 4.2 12.8 1.0
NE E:ARG151 4.4 17.6 1.0
CA E:PRO147 4.6 15.3 1.0
CA D:GLY53 4.6 15.5 1.0
O3A D:08T401 4.6 15.2 1.0
ND2 D:ASN139 4.6 24.6 1.0
OD1 D:ASN139 4.6 27.1 1.0
OE2 D:GLU108 4.7 53.2 1.0
NZ D:LYS56 4.7 19.3 1.0
NE D:ARG205 4.7 24.2 1.0
CG D:PRO52 4.8 9.5 1.0
O E:PRO147 4.9 26.5 1.0
CG E:PRO147 4.9 14.1 1.0

Fluorine binding site 9 out of 9 in 8uh7

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Fluorine binding site 9 out of 9 in the Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 9 of Structure of T4 Bacteriophage Clamp Loader Bound to the T4 Clamp, Primer-Template Dna, and Atp Analog within 5.0Å range:
probe atom residue distance (Å) B Occ
D:F401

b:18.3
occ:1.00
F3 D:08T401 0.0 18.3 1.0
BE D:08T401 1.5 21.5 1.0
F2 D:08T401 2.4 18.7 1.0
F1 D:08T401 2.5 21.4 1.0
O3B D:08T401 2.6 16.5 1.0
ND2 D:ASN139 2.7 24.6 1.0
NZ D:LYS56 2.9 19.3 1.0
OD1 D:ASN139 3.3 27.1 1.0
CG D:ASN139 3.4 21.3 1.0
CA D:PRO52 3.6 14.6 1.0
CE D:LYS56 3.6 18.5 1.0
PB D:08T401 3.8 24.9 1.0
O D:HOH508 3.8 23.4 1.0
CB D:PRO52 3.9 16.9 1.0
O2B D:08T401 4.0 25.2 1.0
N D:GLY53 4.0 18.5 1.0
OE2 D:GLU108 4.2 53.2 1.0
OE1 D:GLU108 4.3 54.5 1.0
O1B D:08T401 4.3 30.8 1.0
C D:PRO52 4.4 19.1 1.0
MG D:MG402 4.4 11.3 1.0
CD D:LYS56 4.5 13.9 1.0
NH2 E:ARG151 4.6 25.2 1.0
NH1 E:ARG151 4.6 14.8 1.0
N D:PRO52 4.6 10.6 1.0
CD D:GLU108 4.7 62.7 1.0
O D:SER51 4.7 27.3 1.0
CB D:ASN139 4.8 12.8 1.0
NH2 D:ARG205 5.0 27.7 1.0
O D:SER49 5.0 24.1 1.0

Reference:

K.Marcus, Y.Huang, S.Subramanian, C.L.Gee, K.Gorday, S.Ghaffari-Kashani, X.R.Luo, L.Zheng, M.O'donnell, S.Subramaniam, J.Kuriyan. Autoinhibition of A Clamp-Loader Atpase Revealed By Deep Mutagenesis and Cryo-Em To Be Published.
Page generated: Wed Jul 16 09:16:41 2025

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