Fluorine in PDB 1rm9: Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code

The structure of Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code, PDB code: 1rm9 was solved by N.Budisa, P.P.Pal, S.Alefelder, P.Birle, T.Krywcun, M.Rubini, W.Wenger, J.H.Bae, T.Steiner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.057, 62.507, 69.466, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / 26.8

The binding sites of Fluorine atom in the Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code (pdb code 1rm9). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 2 binding sites of Fluorine where determined in the Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code, PDB code: 1rm9:
Jump to Fluorine binding site number: 1; 2;

Fluorine binding site 1 out of 2 in the Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code within 5.0Å range: probe atom residue distance (Å) B Occ A:F57 b:15.8 occ:1.00 F A:4FW57 0.0 15.8 1.0 CE3 A:4FW57 1.3 15.2 1.0 CZ3 A:4FW57 2.3 14.3 1.0 CD2 A:4FW57 2.4 16.7 1.0 CG A:4FW57 3.1 17.2 1.0 CB A:4FW57 3.2 18.2 1.0 CA A:4FW57 3.2 19.3 1.0 O A:4FW57 3.3 19.0 1.0 CE A:MET218 3.5 21.3 1.0 C A:4FW57 3.6 18.4 1.0 CD1 A:LEU64 3.6 16.6 1.0 CH2 A:4FW57 3.6 14.2 1.0 CE2 A:4FW57 3.7 16.0 1.0 CB A:LEU60 3.9 11.8 1.0 SD A:MET218 3.9 20.2 1.0 CD2 A:LEU53 4.1 22.4 1.0 CZ2 A:4FW57 4.2 14.8 1.0 CD1 A:LEU60 4.2 13.1 1.0 CD1 A:4FW57 4.4 17.8 1.0 CB A:MET218 4.4 17.3 1.0 CG A:LEU60 4.5 13.8 1.0 N A:4FW57 4.6 21.6 1.0 NE1 A:4FW57 4.6 16.5 1.0 CG A:MET218 4.8 18.2 1.0 N A:PRO58 4.8 17.8 1.0 CA A:LEU60 4.8 12.2 1.0 C A:LEU60 4.9 12.4 1.0 CG A:LEU64 5.0 17.2 1.0

Fluorine binding site 2 out of 2 in the Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code within 5.0Å range: probe atom residue distance (Å) B Occ A:F66 b:37.0 occ:1.00 F10 A:4F366 0.0 37.0 1.0 C2 A:4F366 1.3 36.0 1.0 C1 A:4F366 2.3 36.7 1.0 C3 A:4F366 2.4 38.8 1.0 CG2 A:VAL150 2.9 23.0 1.0 C7 A:4F366 3.1 35.8 1.0 C11 A:4F366 3.2 36.4 1.0 CZ A:PHE165 3.2 20.6 1.0 CE2 A:PHE165 3.3 20.6 1.0 O A:HOH271 3.5 15.3 1.0 C6 A:4F366 3.6 36.8 1.0 NE2 A:GLN69 3.7 22.5 1.0 C4 A:4F366 3.7 39.0 1.0 CE1 A:PHE165 3.9 21.8 1.0 CD2 A:PHE165 4.0 21.1 1.0 CG1 A:VAL150 4.1 23.9 1.0 CB A:VAL150 4.1 24.4 1.0 C5 A:4F366 4.1 39.1 1.0 NH2 A:ARG96 4.3 17.0 1.0 OG1 A:THR203 4.3 11.5 1.0 C8 A:4F366 4.3 37.1 1.0 CD1 A:PHE165 4.5 19.9 1.0 CG A:PHE165 4.5 21.4 1.0 N9 A:4F366 4.6 40.3 1.0 C12 A:4F366 4.6 35.2 1.0 CG2 A:THR62 4.6 14.9 1.0 CB A:THR62 4.8 14.4 1.0 CB A:THR203 4.9 14.2 1.0 CD A:GLN69 4.9 22.4 1.0 CA A:VAL150 5.0 24.5 1.0

N.Budisa, P.P.Pal, S.Alefelder, P.Birle, T.Krywcun, M.Rubini, W.Wenger, J.H.Bae, T.Steiner. Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with An Expanded Genetic Code Biol.Chem. V. 385 191 2004.
ISSN: ISSN 1431-6730
PubMed: 15101562
DOI: 10.1515/BC.2004.038