Fluorine in PDB 1svk: Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
Enzymatic activity of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
All present enzymatic activity of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp:
3.6.1.46;
Protein crystallography data
The structure of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp, PDB code: 1svk
was solved by
C.J.Thomas,
X.Du,
P.Li,
Y.Wang,
E.M.Ross,
S.R.Sprang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
20.00 /
2.00
|
Space group
|
P 32 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
79.473,
79.473,
105.209,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
19.7 /
23.9
|
Other elements in 1svk:
The structure of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp also contains other interesting chemical elements:
Fluorine Binding Sites:
The binding sites of Fluorine atom in the Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
(pdb code 1svk). This binding sites where shown within
5.0 Angstroms radius around Fluorine atom.
In total 4 binding sites of Fluorine where determined in the
Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp, PDB code: 1svk:
Jump to Fluorine binding site number:
1;
2;
3;
4;
Fluorine binding site 1 out
of 4 in 1svk
Go back to
Fluorine Binding Sites List in 1svk
Fluorine binding site 1 out
of 4 in the Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 1 of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F357
b:17.5
occ:1.00
|
F1
|
A:ALF357
|
0.0
|
17.5
|
1.0
|
AL
|
A:ALF357
|
1.7
|
18.0
|
1.0
|
F2
|
A:ALF357
|
2.5
|
22.1
|
1.0
|
F4
|
A:ALF357
|
2.5
|
16.5
|
1.0
|
NE2
|
A:GLN204
|
2.6
|
26.2
|
1.0
|
O3B
|
A:GDP355
|
2.6
|
15.7
|
1.0
|
O
|
A:HOH736
|
2.7
|
17.1
|
1.0
|
N
|
A:GLU43
|
2.9
|
15.3
|
1.0
|
NH2
|
A:ARG178
|
3.1
|
15.9
|
1.0
|
CA
|
A:GLY42
|
3.2
|
14.4
|
1.0
|
CD
|
A:GLN204
|
3.4
|
28.4
|
1.0
|
OE1
|
A:GLN204
|
3.4
|
30.2
|
1.0
|
F3
|
A:ALF357
|
3.5
|
19.1
|
1.0
|
C
|
A:GLY42
|
3.5
|
15.4
|
1.0
|
NH1
|
A:ARG178
|
3.7
|
14.1
|
1.0
|
CZ
|
A:ARG178
|
3.8
|
21.0
|
1.0
|
CG
|
A:GLU43
|
4.0
|
25.1
|
1.0
|
CA
|
A:GLU43
|
4.0
|
17.2
|
1.0
|
PB
|
A:GDP355
|
4.1
|
15.8
|
1.0
|
O
|
A:HOH410
|
4.5
|
24.6
|
1.0
|
N
|
A:GLY203
|
4.5
|
14.8
|
1.0
|
CB
|
A:GLU43
|
4.5
|
20.4
|
1.0
|
N
|
A:GLY42
|
4.6
|
15.0
|
1.0
|
O
|
A:GLY42
|
4.7
|
16.8
|
1.0
|
O1B
|
A:GDP355
|
4.8
|
15.6
|
1.0
|
CG
|
A:GLN204
|
4.8
|
22.5
|
1.0
|
O
|
A:HOH562
|
4.8
|
33.7
|
1.0
|
O3A
|
A:GDP355
|
4.9
|
19.1
|
1.0
|
N
|
A:THR181
|
4.9
|
17.3
|
1.0
|
N
|
A:GLN204
|
5.0
|
18.1
|
1.0
|
O2B
|
A:GDP355
|
5.0
|
15.4
|
1.0
|
|
Fluorine binding site 2 out
of 4 in 1svk
Go back to
Fluorine Binding Sites List in 1svk
Fluorine binding site 2 out
of 4 in the Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 2 of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F357
b:22.1
occ:1.00
|
F2
|
A:ALF357
|
0.0
|
22.1
|
1.0
|
AL
|
A:ALF357
|
1.8
|
18.0
|
1.0
|
F1
|
A:ALF357
|
2.5
|
17.5
|
1.0
|
F3
|
A:ALF357
|
2.5
|
19.1
|
1.0
|
O3B
|
A:GDP355
|
2.6
|
15.7
|
1.0
|
NH1
|
A:ARG178
|
2.7
|
14.1
|
1.0
|
O
|
A:HOH736
|
2.7
|
17.1
|
1.0
|
N
|
A:THR181
|
2.8
|
17.3
|
1.0
|
O
|
A:HOH359
|
3.0
|
17.5
|
1.0
|
CA
|
A:PRO180
|
3.2
|
19.7
|
1.0
|
NH2
|
A:ARG178
|
3.2
|
15.9
|
1.0
|
CZ
|
A:ARG178
|
3.3
|
21.0
|
1.0
|
C
|
A:PRO180
|
3.5
|
21.2
|
1.0
|
MG
|
A:MG356
|
3.5
|
15.7
|
1.0
|
F4
|
A:ALF357
|
3.5
|
16.5
|
1.0
|
OG1
|
A:THR181
|
3.8
|
19.2
|
1.0
|
PB
|
A:GDP355
|
3.9
|
15.8
|
1.0
|
CA
|
A:THR181
|
3.9
|
17.5
|
1.0
|
O
|
A:VAL179
|
4.0
|
21.2
|
1.0
|
CB
|
A:THR181
|
4.0
|
14.7
|
1.0
|
O2B
|
A:GDP355
|
4.0
|
15.4
|
1.0
|
CB
|
A:PRO180
|
4.1
|
21.3
|
1.0
|
N
|
A:PRO180
|
4.2
|
21.0
|
1.0
|
O2A
|
A:GDP355
|
4.4
|
21.3
|
1.0
|
O
|
A:THR181
|
4.4
|
18.4
|
1.0
|
C
|
A:VAL179
|
4.5
|
20.5
|
1.0
|
NE2
|
A:GLN204
|
4.5
|
26.2
|
1.0
|
NE
|
A:ARG178
|
4.6
|
20.4
|
1.0
|
OE1
|
A:GLN204
|
4.6
|
30.2
|
1.0
|
O3A
|
A:GDP355
|
4.6
|
19.1
|
1.0
|
C
|
A:THR181
|
4.7
|
22.1
|
1.0
|
O
|
A:PRO180
|
4.7
|
20.8
|
1.0
|
N
|
A:GLU43
|
4.8
|
15.3
|
1.0
|
CG
|
A:PRO180
|
4.8
|
22.7
|
1.0
|
|
Fluorine binding site 3 out
of 4 in 1svk
Go back to
Fluorine Binding Sites List in 1svk
Fluorine binding site 3 out
of 4 in the Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 3 of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F357
b:19.1
occ:1.00
|
F3
|
A:ALF357
|
0.0
|
19.1
|
1.0
|
AL
|
A:ALF357
|
1.7
|
18.0
|
1.0
|
MG
|
A:MG356
|
2.0
|
15.7
|
1.0
|
F4
|
A:ALF357
|
2.4
|
16.5
|
1.0
|
F2
|
A:ALF357
|
2.5
|
22.1
|
1.0
|
O3B
|
A:GDP355
|
2.6
|
15.7
|
1.0
|
O
|
A:HOH736
|
2.7
|
17.1
|
1.0
|
O
|
A:HOH358
|
2.8
|
15.9
|
1.0
|
OG1
|
A:THR181
|
2.9
|
19.2
|
1.0
|
O2B
|
A:GDP355
|
2.9
|
15.4
|
1.0
|
CB
|
A:THR181
|
3.0
|
14.7
|
1.0
|
PB
|
A:GDP355
|
3.2
|
15.8
|
1.0
|
O
|
A:HOH359
|
3.3
|
17.5
|
1.0
|
F1
|
A:ALF357
|
3.5
|
17.5
|
1.0
|
N
|
A:THR181
|
3.6
|
17.3
|
1.0
|
O
|
A:VAL201
|
3.7
|
16.9
|
1.0
|
CA
|
A:THR181
|
3.8
|
17.5
|
1.0
|
CA
|
A:GLY202
|
3.9
|
16.7
|
1.0
|
O1B
|
A:GDP355
|
4.0
|
15.6
|
1.0
|
OG
|
A:SER47
|
4.1
|
14.5
|
0.8
|
NZ
|
A:LYS46
|
4.1
|
14.7
|
1.0
|
CE
|
A:LYS46
|
4.1
|
11.7
|
1.0
|
O
|
A:THR181
|
4.1
|
18.4
|
1.0
|
CG2
|
A:THR181
|
4.2
|
19.2
|
1.0
|
N
|
A:GLY203
|
4.3
|
14.8
|
1.0
|
C
|
A:VAL201
|
4.5
|
17.1
|
1.0
|
C
|
A:THR181
|
4.5
|
22.1
|
1.0
|
C
|
A:GLY202
|
4.6
|
18.7
|
1.0
|
O3A
|
A:GDP355
|
4.6
|
19.1
|
1.0
|
N
|
A:GLY202
|
4.6
|
17.4
|
1.0
|
NH1
|
A:ARG178
|
4.7
|
14.1
|
1.0
|
C
|
A:PRO180
|
4.8
|
21.2
|
1.0
|
|
Fluorine binding site 4 out
of 4 in 1svk
Go back to
Fluorine Binding Sites List in 1svk
Fluorine binding site 4 out
of 4 in the Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 4 of Structure of the K180P Mutant of Gi Alpha Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F357
b:16.5
occ:1.00
|
F4
|
A:ALF357
|
0.0
|
16.5
|
1.0
|
AL
|
A:ALF357
|
1.7
|
18.0
|
1.0
|
F3
|
A:ALF357
|
2.4
|
19.1
|
1.0
|
F1
|
A:ALF357
|
2.5
|
17.5
|
1.0
|
O3B
|
A:GDP355
|
2.6
|
15.7
|
1.0
|
O
|
A:HOH736
|
2.6
|
17.1
|
1.0
|
N
|
A:GLY203
|
2.8
|
14.8
|
1.0
|
NZ
|
A:LYS46
|
2.8
|
14.7
|
1.0
|
CA
|
A:GLY42
|
3.3
|
14.4
|
1.0
|
CA
|
A:GLY203
|
3.4
|
14.1
|
1.0
|
PB
|
A:GDP355
|
3.5
|
15.8
|
1.0
|
F2
|
A:ALF357
|
3.5
|
22.1
|
1.0
|
N
|
A:GLU43
|
3.6
|
15.3
|
1.0
|
CE
|
A:LYS46
|
3.6
|
11.7
|
1.0
|
O1B
|
A:GDP355
|
3.6
|
15.6
|
1.0
|
C
|
A:GLY202
|
3.7
|
18.7
|
1.0
|
CA
|
A:GLY202
|
3.9
|
16.7
|
1.0
|
C
|
A:GLY42
|
3.9
|
15.4
|
1.0
|
O
|
A:ALA41
|
3.9
|
15.9
|
1.0
|
OE1
|
A:GLN204
|
4.0
|
30.2
|
1.0
|
N
|
A:GLY42
|
4.0
|
15.0
|
1.0
|
O2B
|
A:GDP355
|
4.2
|
15.4
|
1.0
|
NE2
|
A:GLN204
|
4.2
|
26.2
|
1.0
|
O
|
A:VAL201
|
4.3
|
16.9
|
1.0
|
MG
|
A:MG356
|
4.3
|
15.7
|
1.0
|
C
|
A:ALA41
|
4.3
|
17.4
|
1.0
|
C
|
A:GLY203
|
4.4
|
16.9
|
1.0
|
CD
|
A:GLN204
|
4.5
|
28.4
|
1.0
|
N
|
A:GLN204
|
4.5
|
18.1
|
1.0
|
O
|
A:HOH358
|
4.5
|
15.9
|
1.0
|
O
|
A:GLY40
|
4.8
|
14.9
|
1.0
|
CA
|
A:GLU43
|
4.8
|
17.2
|
1.0
|
O
|
A:GLY202
|
4.8
|
17.4
|
1.0
|
O3A
|
A:GDP355
|
4.9
|
19.1
|
1.0
|
N
|
A:GLY202
|
5.0
|
17.4
|
1.0
|
|
Reference:
C.J.Thomas,
X.Du,
P.Li,
Y.Wang,
E.M.Ross,
S.R.Sprang.
Uncoupling Conformational Change From Gtp Hydrolysis in A Heterotrimeric G Protein {Alpha}-Subunit. Proc.Natl.Acad.Sci.Usa V. 101 7560 2004.
ISSN: ISSN 0027-8424
PubMed: 15128951
DOI: 10.1073/PNAS.0304091101
Page generated: Wed Jul 31 12:53:19 2024
|