Fluorine in PDB 2g83: Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit

The structure of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit, PDB code: 2g83 was solved by C.A.Johnston, J.K.Ramer, R.Blaesius, B.Kuhlman, V.Y.Arshavsky, D.P.Siderovski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	103.130, 103.130, 206.990, 90.00, 90.00, 120.00
R / Rfree (%)	26.9 / 30

The structure of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Aluminium	(Al)	2 atoms

The binding sites of Fluorine atom in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit (pdb code 2g83). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 8 binding sites of Fluorine where determined in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit, PDB code: 2g83:
Jump to Fluorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Fluorine binding site 1 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:F357 b:55.6 occ:1.00 F1 A:ALF357 0.0 55.6 1.0 AL A:ALF357 1.7 56.2 1.0 F4 A:ALF357 2.2 54.9 1.0 F2 A:ALF357 2.2 53.6 1.0 O3B A:GDP355 2.4 34.7 1.0 OG1 A:THR181 2.4 42.1 1.0 MG A:MG356 2.6 29.3 1.0 CB A:THR181 2.9 42.6 1.0 F3 A:ALF357 3.1 53.9 1.0 O A:HOH2 3.2 44.1 1.0 PB A:GDP355 3.2 39.4 1.0 N A:THR181 3.4 37.6 1.0 O1B A:GDP355 3.4 39.6 1.0 O2B A:GDP355 3.6 37.6 1.0 CA A:THR181 3.7 37.7 1.0 O A:THR181 3.7 41.9 1.0 O A:VAL201 3.8 30.8 1.0 OG A:SER47 3.8 26.1 1.0 CA A:GLY202 3.9 37.7 1.0 CG2 A:THR181 4.0 42.9 1.0 C A:THR181 4.1 39.7 1.0 NH1 A:ARG178 4.1 37.5 1.0 NZ A:LYS46 4.2 43.6 1.0 N A:GLY203 4.3 61.1 1.0 C A:LYS180 4.5 30.5 1.0 CE A:LYS46 4.6 43.5 1.0 C A:VAL201 4.6 29.4 1.0 N A:GLY202 4.6 36.1 1.0 C A:GLY202 4.6 39.7 1.0 O3A A:GDP355 4.6 39.7 1.0 CA A:LYS180 4.7 30.3 1.0 NH2 A:ARG178 4.8 34.9 1.0 OE1 A:GLN204 4.9 51.8 1.0 CZ A:ARG178 4.9 36.9 1.0

Fluorine binding site 2 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:F357 b:53.6 occ:1.00 F2 A:ALF357 0.0 53.6 1.0 AL A:ALF357 1.7 56.2 1.0 F3 A:ALF357 2.2 53.9 1.0 F1 A:ALF357 2.2 55.6 1.0 O1B A:GDP355 2.9 39.6 1.0 NZ A:LYS46 3.0 43.6 1.0 O2B A:GDP355 3.1 37.6 1.0 F4 A:ALF357 3.1 54.9 1.0 N A:GLU43 3.2 41.0 1.0 PB A:GDP355 3.3 39.4 1.0 O3B A:GDP355 3.4 34.7 1.0 N A:GLY203 3.4 61.1 1.0 CA A:GLY42 3.5 37.9 1.0 C A:GLY42 3.9 38.6 1.0 O A:ALA41 3.9 39.4 1.0 CE A:LYS46 4.0 43.5 1.0 CA A:GLY203 4.1 63.1 1.0 OE1 A:GLN204 4.2 51.8 1.0 CA A:GLY202 4.2 37.7 1.0 CA A:GLU43 4.3 40.0 1.0 MG A:MG356 4.3 29.3 1.0 C A:GLY202 4.3 39.7 1.0 NE2 A:GLN204 4.3 50.2 1.0 NH1 A:ARG178 4.4 37.5 1.0 O A:HOH2 4.5 44.1 1.0 N A:GLY42 4.5 37.6 1.0 C A:ALA41 4.5 39.6 1.0 OG1 A:THR181 4.5 42.1 1.0 O3A A:GDP355 4.6 39.7 1.0 CD A:GLN204 4.6 50.5 1.0 O A:VAL201 4.6 30.8 1.0 NH2 A:ARG178 4.7 34.9 1.0 N A:SER44 4.9 37.5 1.0 N A:GLN204 4.9 60.3 1.0 O A:GLY42 5.0 38.7 1.0 C A:GLY203 5.0 64.6 1.0

Fluorine binding site 3 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:F357 b:53.9 occ:1.00 F3 A:ALF357 0.0 53.9 1.0 AL A:ALF357 1.7 56.2 1.0 F2 A:ALF357 2.2 53.6 1.0 F4 A:ALF357 2.2 54.9 1.0 NH2 A:ARG178 2.6 34.9 1.0 O1B A:GDP355 2.9 39.6 1.0 NH1 A:ARG178 2.9 37.5 1.0 N A:GLU43 2.9 41.0 1.0 F1 A:ALF357 3.1 55.6 1.0 CZ A:ARG178 3.1 36.9 1.0 NE2 A:GLN204 3.2 50.2 1.0 CA A:GLU43 3.7 40.0 1.0 C A:GLY42 3.7 38.6 1.0 CA A:GLY42 3.8 37.9 1.0 PB A:GDP355 3.9 39.4 1.0 OE1 A:GLN204 3.9 51.8 1.0 CD A:GLN204 3.9 50.5 1.0 CG A:GLU43 4.1 48.9 1.0 O3B A:GDP355 4.1 34.7 1.0 CB A:GLU43 4.2 44.1 1.0 O2B A:GDP355 4.3 37.6 1.0 CG A:LYS180 4.3 36.0 1.0 NE A:ARG178 4.4 35.8 1.0 MG A:MG356 4.5 29.3 1.0 O A:GLY42 4.8 38.7 1.0 CD A:GLU43 4.8 51.7 1.0 N A:GLY203 4.9 61.1 1.0 O3A A:GDP355 5.0 39.7 1.0 C A:GLU43 5.0 39.7 1.0 OE1 A:GLU43 5.0 53.6 1.0 N A:THR181 5.0 37.6 1.0 CA A:LYS180 5.0 30.3 1.0

Fluorine binding site 4 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:F357 b:54.9 occ:1.00 F4 A:ALF357 0.0 54.9 1.0 AL A:ALF357 1.7 56.2 1.0 F1 A:ALF357 2.2 55.6 1.0 F3 A:ALF357 2.2 53.9 1.0 NH1 A:ARG178 2.5 37.5 1.0 NH2 A:ARG178 2.8 34.9 1.0 N A:THR181 2.9 37.6 1.0 CZ A:ARG178 3.0 36.9 1.0 MG A:MG356 3.0 29.3 1.0 CA A:LYS180 3.1 30.3 1.0 F2 A:ALF357 3.1 53.6 1.0 CG A:LYS180 3.3 36.0 1.0 OG1 A:THR181 3.4 42.1 1.0 O3B A:GDP355 3.4 34.7 1.0 O1B A:GDP355 3.5 39.6 1.0 C A:LYS180 3.5 30.5 1.0 CB A:LYS180 3.7 35.1 1.0 PB A:GDP355 3.9 39.4 1.0 CB A:THR181 3.9 42.6 1.0 CA A:THR181 3.9 37.7 1.0 O A:THR181 4.0 41.9 1.0 O A:VAL179 4.0 41.8 1.0 N A:LYS180 4.1 29.0 1.0 NE A:ARG178 4.2 35.8 1.0 C A:VAL179 4.4 40.7 1.0 C A:THR181 4.4 39.7 1.0 NE2 A:GLN204 4.5 50.2 1.0 CD A:LYS180 4.6 38.6 1.0 OE1 A:GLN204 4.6 51.8 1.0 O A:LYS180 4.6 30.9 1.0 O2B A:GDP355 4.7 37.6 1.0 O2A A:GDP355 4.8 40.0 1.0 OG A:SER47 4.8 26.1 1.0 N A:GLU43 4.9 41.0 1.0 CD A:ARG178 5.0 33.5 1.0

Fluorine binding site 5 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 5 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:F359 b:62.9 occ:1.00 F1 B:ALF359 0.0 62.9 1.0 AL B:ALF359 1.7 61.6 1.0 F2 B:ALF359 2.2 59.9 1.0 F4 B:ALF359 2.3 65.1 1.0 NE2 B:GLN204 2.7 38.5 1.0 O1B B:GDP355 2.8 44.3 1.0 OE1 B:GLN204 2.8 38.2 1.0 CD B:GLN204 3.1 39.5 1.0 F3 B:ALF359 3.1 63.7 1.0 N B:GLU43 3.1 49.3 1.0 CA B:GLY42 3.2 65.0 1.0 NH2 B:ARG178 3.2 55.4 1.0 C B:GLY42 3.6 65.0 1.0 CG B:GLU43 4.1 51.4 1.0 CZ B:ARG178 4.1 54.5 1.0 PB B:GDP355 4.1 42.4 1.0 NH1 B:ARG178 4.1 54.3 1.0 N B:GLY203 4.1 63.0 1.0 CA B:GLU43 4.2 47.9 1.0 CG B:GLN204 4.4 37.4 1.0 N B:GLY42 4.5 64.7 1.0 O2B B:GDP355 4.5 38.5 1.0 O B:GLY203 4.6 63.3 1.0 C B:GLY203 4.7 63.3 1.0 O B:GLY42 4.7 65.7 1.0 CA B:GLY203 4.7 63.2 1.0 CB B:GLU43 4.7 46.2 1.0 O B:ALA41 4.8 36.8 1.0 CG B:LYS180 4.8 51.7 1.0 MG B:MG358 4.9 45.1 1.0 C B:ALA41 5.0 37.6 1.0

Fluorine binding site 6 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 6 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:F359 b:59.9 occ:1.00 F2 B:ALF359 0.0 59.9 1.0 O1B B:GDP355 1.3 44.3 1.0 AL B:ALF359 1.7 61.6 1.0 F3 B:ALF359 2.1 63.7 1.0 F1 B:ALF359 2.2 62.9 1.0 PB B:GDP355 2.4 42.4 1.0 O2B B:GDP355 2.8 38.5 1.0 F4 B:ALF359 3.1 65.1 1.0 O3B B:GDP355 3.1 39.1 1.0 N B:GLU43 3.3 49.3 1.0 NZ B:LYS46 3.5 48.5 1.0 MG B:MG358 3.6 45.1 1.0 O3A B:GDP355 3.7 45.5 1.0 O B:HOH1 3.9 35.6 1.0 CA B:GLY42 3.9 65.0 1.0 N B:GLY203 4.0 63.0 1.0 CE B:LYS46 4.1 48.0 1.0 CA B:GLU43 4.1 47.9 1.0 C B:GLY42 4.1 65.0 1.0 OG1 B:THR181 4.2 41.8 1.0 O B:ALA41 4.2 36.8 1.0 NH1 B:ARG178 4.3 54.3 1.0 OE1 B:GLN204 4.4 38.2 1.0 NH2 B:ARG178 4.5 55.4 1.0 CA B:GLY202 4.5 33.9 1.0 OG B:SER47 4.5 41.2 1.0 CA B:GLY203 4.7 63.2 1.0 O B:VAL201 4.7 37.4 1.0 C B:GLY202 4.7 35.4 1.0 CB B:THR181 4.7 41.7 1.0 NE2 B:GLN204 4.8 38.5 1.0 N B:SER44 4.8 45.8 1.0 C B:ALA41 4.8 37.6 1.0 CZ B:ARG178 4.9 54.5 1.0 N B:GLY42 4.9 64.7 1.0 CD B:GLN204 5.0 39.5 1.0

Fluorine binding site 7 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 7 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:F359 b:63.7 occ:1.00 F3 B:ALF359 0.0 63.7 1.0 AL B:ALF359 1.7 61.6 1.0 F2 B:ALF359 2.1 59.9 1.0 F4 B:ALF359 2.2 65.1 1.0 O1B B:GDP355 2.7 44.3 1.0 OG1 B:THR181 2.8 41.8 1.0 CB B:THR181 2.8 41.7 1.0 CA B:GLY202 3.0 33.9 1.0 O B:HOH1 3.1 35.6 1.0 F1 B:ALF359 3.1 62.9 1.0 MG B:MG358 3.2 45.1 1.0 O B:VAL201 3.5 37.4 1.0 O B:THR181 3.5 45.1 1.0 N B:GLY203 3.6 63.0 1.0 N B:THR181 3.6 46.8 1.0 O3B B:GDP355 3.7 39.1 1.0 CA B:THR181 3.7 44.8 1.0 PB B:GDP355 3.7 42.4 1.0 C B:GLY202 3.8 35.4 1.0 CG2 B:THR181 3.9 39.5 1.0 N B:GLY202 4.0 34.8 1.0 C B:THR181 4.0 45.0 1.0 C B:VAL201 4.1 36.5 1.0 OG B:SER47 4.2 41.2 1.0 OE1 B:GLN204 4.2 38.2 1.0 NZ B:LYS46 4.4 48.5 1.0 CE B:LYS46 4.5 48.0 1.0 O2B B:GDP355 4.5 38.5 1.0 CA B:GLY203 4.8 63.2 1.0 C B:LYS180 4.8 46.6 1.0 O B:GLY202 4.9 33.4 1.0

Fluorine binding site 8 out of 8 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 8 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:F359 b:65.1 occ:1.00 F4 B:ALF359 0.0 65.1 1.0 AL B:ALF359 1.7 61.6 1.0 F3 B:ALF359 2.2 63.7 1.0 F1 B:ALF359 2.3 62.9 1.0 OE1 B:GLN204 2.3 38.2 1.0 F2 B:ALF359 3.1 59.9 1.0 O B:THR181 3.1 45.1 1.0 CD B:GLN204 3.3 39.5 1.0 CG B:LYS180 3.5 51.7 1.0 N B:THR181 3.5 46.8 1.0 NE2 B:GLN204 3.6 38.5 1.0 N B:GLY203 3.6 63.0 1.0 CA B:GLY202 3.7 33.9 1.0 O1B B:GDP355 3.7 44.3 1.0 C B:THR181 3.9 45.0 1.0 CB B:THR181 4.0 41.7 1.0 CA B:THR181 4.1 44.8 1.0 C B:GLY202 4.1 35.4 1.0 NH2 B:ARG178 4.2 55.4 1.0 CD B:LYS180 4.2 53.0 1.0 OG1 B:THR181 4.4 41.8 1.0 C B:LYS180 4.4 46.6 1.0 CE B:LYS180 4.4 56.8 1.0 CA B:LYS180 4.4 47.2 1.0 CB B:LYS180 4.5 50.3 1.0 CG B:GLN204 4.5 37.4 1.0 MG B:MG358 4.5 45.1 1.0 CB B:GLN204 4.7 39.6 1.0 CA B:GLY203 4.8 63.2 1.0 N B:GLY202 5.0 34.8 1.0

C.A.Johnston, E.S.Lobanova, A.S.Shavkunov, J.Low, J.K.Ramer, R.Blaesius, Z.Fredericks, F.S.Willard, B.Kuhlman, V.Y.Arshavsky, D.P.Siderovski. Minimal Determinants For Binding Activated Galpha From the Structure of A Galpha(I1)-Peptide Dimer. Biochemistry V. 45 11390 2006.
ISSN: ISSN 0006-2960
PubMed: 16981699
DOI: 10.1021/BI0613832