Fluorine in PDB 2zjy: Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
Enzymatic activity of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
All present enzymatic activity of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp:
3.6.5.1;
Protein crystallography data
The structure of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp, PDB code: 2zjy
was solved by
T.Morikawa,
A.Muroya,
S.Sugio,
K.Wakamatsu,
T.Kohno,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
28.78 /
2.80
|
Space group
|
P 32 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
79.375,
79.375,
105.297,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
18.1 /
26
|
Other elements in 2zjy:
The structure of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp also contains other interesting chemical elements:
Fluorine Binding Sites:
The binding sites of Fluorine atom in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
(pdb code 2zjy). This binding sites where shown within
5.0 Angstroms radius around Fluorine atom.
In total 4 binding sites of Fluorine where determined in the
Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp, PDB code: 2zjy:
Jump to Fluorine binding site number:
1;
2;
3;
4;
Fluorine binding site 1 out
of 4 in 2zjy
Go back to
Fluorine Binding Sites List in 2zjy
Fluorine binding site 1 out
of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 1 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F356
b:8.6
occ:1.00
|
F1
|
A:ALF356
|
0.0
|
8.6
|
1.0
|
AL
|
A:ALF356
|
1.8
|
2.2
|
1.0
|
F2
|
A:ALF356
|
2.1
|
2.2
|
1.0
|
NH2
|
A:ARG178
|
2.7
|
12.7
|
1.0
|
F4
|
A:ALF356
|
2.8
|
30.2
|
1.0
|
NE2
|
A:GLN204
|
2.8
|
22.9
|
1.0
|
O3B
|
A:GDP355
|
2.8
|
22.1
|
1.0
|
N
|
A:GLU43
|
3.1
|
8.0
|
1.0
|
F3
|
A:ALF356
|
3.5
|
25.1
|
1.0
|
CD
|
A:GLN204
|
3.7
|
21.8
|
1.0
|
CZ
|
A:ARG178
|
3.7
|
15.2
|
1.0
|
OE1
|
A:GLN204
|
3.7
|
15.4
|
1.0
|
CA
|
A:GLU43
|
3.9
|
6.8
|
1.0
|
NH1
|
A:ARG178
|
3.9
|
18.9
|
1.0
|
CA
|
A:GLY42
|
4.0
|
12.9
|
1.0
|
C
|
A:GLY42
|
4.0
|
11.3
|
1.0
|
PB
|
A:GDP355
|
4.0
|
18.1
|
1.0
|
CG
|
A:GLU43
|
4.1
|
5.5
|
1.0
|
O1B
|
A:GDP355
|
4.1
|
19.2
|
1.0
|
O
|
A:HOH533
|
4.4
|
2.2
|
1.0
|
CB
|
A:GLU43
|
4.5
|
8.2
|
1.0
|
N
|
A:THR181
|
4.6
|
16.8
|
1.0
|
CG
|
A:LYS180
|
4.7
|
24.6
|
1.0
|
CA
|
A:LYS180
|
4.8
|
11.8
|
1.0
|
N
|
A:GLY203
|
4.9
|
8.3
|
1.0
|
NE
|
A:ARG178
|
4.9
|
17.1
|
1.0
|
O
|
A:HOH424
|
5.0
|
11.9
|
1.0
|
MG
|
A:MG357
|
5.0
|
2.6
|
1.0
|
|
Fluorine binding site 2 out
of 4 in 2zjy
Go back to
Fluorine Binding Sites List in 2zjy
Fluorine binding site 2 out
of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 2 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F356
b:2.2
occ:1.00
|
F2
|
A:ALF356
|
0.0
|
2.2
|
1.0
|
AL
|
A:ALF356
|
1.8
|
2.2
|
1.0
|
F1
|
A:ALF356
|
2.1
|
8.6
|
1.0
|
F3
|
A:ALF356
|
2.5
|
25.1
|
1.0
|
N
|
A:THR181
|
2.8
|
16.8
|
1.0
|
O3B
|
A:GDP355
|
3.0
|
22.1
|
1.0
|
NE2
|
A:GLN204
|
3.1
|
22.9
|
1.0
|
NH2
|
A:ARG178
|
3.2
|
12.7
|
1.0
|
O
|
A:HOH533
|
3.2
|
2.2
|
1.0
|
F4
|
A:ALF356
|
3.4
|
30.2
|
1.0
|
CB
|
A:THR181
|
3.4
|
12.9
|
1.0
|
CA
|
A:THR181
|
3.5
|
18.6
|
1.0
|
O
|
A:THR181
|
3.5
|
25.8
|
1.0
|
MG
|
A:MG357
|
3.6
|
2.6
|
1.0
|
C
|
A:LYS180
|
3.7
|
11.5
|
1.0
|
CA
|
A:LYS180
|
3.8
|
11.8
|
1.0
|
OG1
|
A:THR181
|
3.9
|
18.5
|
1.0
|
CG
|
A:LYS180
|
3.9
|
24.6
|
1.0
|
C
|
A:THR181
|
4.0
|
21.7
|
1.0
|
PB
|
A:GDP355
|
4.2
|
18.1
|
1.0
|
CD
|
A:GLN204
|
4.4
|
21.8
|
1.0
|
CZ
|
A:ARG178
|
4.4
|
15.2
|
1.0
|
CB
|
A:LYS180
|
4.4
|
13.3
|
1.0
|
CA
|
A:GLY202
|
4.6
|
10.4
|
1.0
|
O2B
|
A:GDP355
|
4.6
|
28.6
|
1.0
|
N
|
A:GLY203
|
4.6
|
8.3
|
1.0
|
O
|
A:VAL179
|
4.6
|
5.8
|
1.0
|
O1B
|
A:GDP355
|
4.7
|
19.2
|
1.0
|
CG2
|
A:THR181
|
4.7
|
11.7
|
1.0
|
O
|
A:LYS180
|
4.9
|
10.5
|
1.0
|
OE1
|
A:GLN204
|
4.9
|
15.4
|
1.0
|
N
|
A:LYS180
|
5.0
|
11.9
|
1.0
|
N
|
A:GLU43
|
5.0
|
8.0
|
1.0
|
|
Fluorine binding site 3 out
of 4 in 2zjy
Go back to
Fluorine Binding Sites List in 2zjy
Fluorine binding site 3 out
of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 3 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F356
b:25.1
occ:1.00
|
F3
|
A:ALF356
|
0.0
|
25.1
|
1.0
|
AL
|
A:ALF356
|
1.8
|
2.2
|
1.0
|
F2
|
A:ALF356
|
2.5
|
2.2
|
1.0
|
F4
|
A:ALF356
|
2.6
|
30.2
|
1.0
|
MG
|
A:MG357
|
2.7
|
2.6
|
1.0
|
O
|
A:VAL201
|
2.9
|
6.8
|
1.0
|
CA
|
A:GLY202
|
3.1
|
10.4
|
1.0
|
N
|
A:GLY203
|
3.1
|
8.3
|
1.0
|
CE
|
A:LYS46
|
3.2
|
16.7
|
1.0
|
NZ
|
A:LYS46
|
3.3
|
4.1
|
1.0
|
C
|
A:GLY202
|
3.4
|
7.8
|
1.0
|
F1
|
A:ALF356
|
3.5
|
8.6
|
1.0
|
CB
|
A:THR181
|
3.5
|
12.9
|
1.0
|
O3B
|
A:GDP355
|
3.5
|
22.1
|
1.0
|
C
|
A:VAL201
|
3.7
|
7.0
|
1.0
|
O2B
|
A:GDP355
|
3.7
|
28.6
|
1.0
|
PB
|
A:GDP355
|
3.8
|
18.1
|
1.0
|
N
|
A:GLY202
|
3.8
|
7.8
|
1.0
|
O
|
A:THR181
|
3.9
|
25.8
|
1.0
|
O1B
|
A:GDP355
|
4.0
|
19.2
|
1.0
|
O
|
A:HOH533
|
4.0
|
2.2
|
1.0
|
NE2
|
A:GLN204
|
4.1
|
22.9
|
1.0
|
OG1
|
A:THR181
|
4.2
|
18.5
|
1.0
|
CG2
|
A:THR181
|
4.2
|
11.7
|
1.0
|
CA
|
A:GLY203
|
4.2
|
11.0
|
1.0
|
CA
|
A:THR181
|
4.4
|
18.6
|
1.0
|
N
|
A:THR181
|
4.4
|
16.8
|
1.0
|
O
|
A:GLY202
|
4.5
|
14.0
|
1.0
|
C
|
A:THR181
|
4.5
|
21.7
|
1.0
|
CD
|
A:LYS46
|
4.7
|
8.0
|
1.0
|
OD1
|
A:ASP200
|
4.8
|
24.2
|
1.0
|
|
Fluorine binding site 4 out
of 4 in 2zjy
Go back to
Fluorine Binding Sites List in 2zjy
Fluorine binding site 4 out
of 4 in the Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp
Mono view
Stereo pair view
|
A full contact list of Fluorine with other atoms in the F binding
site number 4 of Structure of the K349P Mutant of Gi Alpha 1 Subunit Bound to ALF4 and Gdp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:F356
b:30.2
occ:1.00
|
F4
|
A:ALF356
|
0.0
|
30.2
|
1.0
|
AL
|
A:ALF356
|
1.7
|
2.2
|
1.0
|
F3
|
A:ALF356
|
2.6
|
25.1
|
1.0
|
N
|
A:GLY203
|
2.6
|
8.3
|
1.0
|
F1
|
A:ALF356
|
2.8
|
8.6
|
1.0
|
CA
|
A:GLY203
|
2.9
|
11.0
|
1.0
|
NZ
|
A:LYS46
|
2.9
|
4.1
|
1.0
|
CA
|
A:GLY42
|
3.1
|
12.9
|
1.0
|
NE2
|
A:GLN204
|
3.2
|
22.9
|
1.0
|
O
|
A:ALA41
|
3.3
|
12.8
|
1.0
|
F2
|
A:ALF356
|
3.4
|
2.2
|
1.0
|
N
|
A:GLU43
|
3.5
|
8.0
|
1.0
|
C
|
A:GLY203
|
3.6
|
15.5
|
1.0
|
N
|
A:GLY42
|
3.6
|
12.8
|
1.0
|
C
|
A:ALA41
|
3.7
|
10.0
|
1.0
|
C
|
A:GLY202
|
3.7
|
7.8
|
1.0
|
O1B
|
A:GDP355
|
3.8
|
19.2
|
1.0
|
N
|
A:GLN204
|
3.8
|
16.1
|
1.0
|
C
|
A:GLY42
|
3.8
|
11.3
|
1.0
|
CE
|
A:LYS46
|
3.9
|
16.7
|
1.0
|
CD
|
A:GLN204
|
4.0
|
21.8
|
1.0
|
O3B
|
A:GDP355
|
4.0
|
22.1
|
1.0
|
CA
|
A:GLY202
|
4.2
|
10.4
|
1.0
|
O
|
A:GLY40
|
4.3
|
7.0
|
1.0
|
OE1
|
A:GLN204
|
4.4
|
15.4
|
1.0
|
PB
|
A:GDP355
|
4.4
|
18.1
|
1.0
|
O
|
A:GLY203
|
4.5
|
15.3
|
1.0
|
O
|
A:GLY202
|
4.7
|
14.0
|
1.0
|
CA
|
A:GLU43
|
4.8
|
6.8
|
1.0
|
O
|
A:VAL201
|
4.8
|
6.8
|
1.0
|
CA
|
A:ALA41
|
5.0
|
10.4
|
1.0
|
MG
|
A:MG357
|
5.0
|
2.6
|
1.0
|
CG
|
A:GLN204
|
5.0
|
15.0
|
1.0
|
O
|
A:GLY42
|
5.0
|
11.7
|
1.0
|
|
Reference:
T.Morikawa,
A.Muroya,
S.Sugio,
K.Wakamatsu,
T.Kohno.
How Gpcrs Activate G Proteins: Structural Changes Form C-Terminal Tail to Gdp Binding Pocket To Be Published.
Page generated: Wed Jul 31 16:59:05 2024
|