Fluorine in PDB 4f5o: Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog

All present enzymatic activity of Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog:
2.7.7.7;

The structure of Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog, PDB code: 4f5o was solved by B.D.Freudenthal, W.A.Beard, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	22.57 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	54.480, 78.923, 55.134, 90.00, 106.61, 90.00
R / Rfree (%)	20.3 / 26.7

The structure of Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Fluorine atom in the Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog (pdb code 4f5o). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 2 binding sites of Fluorine where determined in the Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog, PDB code: 4f5o:
Jump to Fluorine binding site number: 1; 2;

Fluorine binding site 1 out of 2 in the Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:F401 b:63.8 occ:1.00 F1A A:6CF401 0.0 63.8 1.0 C3A A:6CF401 1.4 63.0 1.0 F2A A:6CF401 2.3 67.0 1.0 P1 A:6CF401 2.5 68.4 1.0 P2 A:6CF401 2.5 65.8 1.0 O5' A:6CF401 2.9 67.6 1.0 O1B A:6CF401 2.9 65.2 1.0 O2A A:6CF401 3.1 60.9 1.0 O2B A:6CF401 3.2 58.9 1.0 HO2B A:6CF401 3.3 70.7 1.0 H3' A:6CF401 3.7 76.9 1.0 O1A A:6CF401 3.9 57.8 1.0 O3B A:6CF401 3.9 59.5 1.0 C5' A:6CF401 4.1 63.5 1.0 H5'A A:6CF401 4.1 76.3 1.0 H6 A:6CF401 4.5 63.0 1.0 HO1A A:6CF401 4.7 69.3 1.0 C3' A:6CF401 4.7 64.1 1.0 H5' A:6CF401 4.7 76.3 1.0 HO3' A:6CF401 5.0 74.1 1.0

Fluorine binding site 2 out of 2 in the Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Open Ternary Complex of R283K Dna Polymerase Beta with A One Metal Bound Dctp Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:F401 b:67.0 occ:1.00 F2A A:6CF401 0.0 67.0 1.0 C3A A:6CF401 1.4 63.0 1.0 F1A A:6CF401 2.3 63.8 1.0 P1 A:6CF401 2.5 68.4 1.0 P2 A:6CF401 2.5 65.8 1.0 O2A A:6CF401 2.8 60.9 1.0 O3B A:6CF401 2.9 59.5 1.0 O1B A:6CF401 3.1 65.2 1.0 O1A A:6CF401 3.3 57.8 1.0 O A:HOH688 3.8 54.6 1.0 O5' A:6CF401 3.9 67.6 1.0 O2B A:6CF401 3.9 58.9 1.0 HO1A A:6CF401 4.1 69.3 1.0 HO2B A:6CF401 4.4 70.7 1.0 O A:HOH700 4.5 49.0 1.0 P3 A:6CF401 4.5 53.6 1.0 O3G A:6CF401 4.9 45.4 1.0 MG A:MG402 5.0 51.0 1.0 C5' A:6CF401 5.0 63.5 1.0

B.D.Freudenthal, W.A.Beard, S.H.Wilson. Structures of Dntp Intermediate States During Dna Polymerase Active Site Assembly. Structure V. 20 1829 2012.
ISSN: ISSN 0969-2126
PubMed: 22959623
DOI: 10.1016/J.STR.2012.08.008