Atomistry » Fluorine » PDB 4j0v-4jps » 4jb4
Atomistry »
  Fluorine »
    PDB 4j0v-4jps »
      4jb4 »

Fluorine in PDB 4jb4: Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility

Enzymatic activity of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility

All present enzymatic activity of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility:
1.11.1.5;

Protein crystallography data

The structure of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility, PDB code: 4jb4 was solved by A.C.Wohlkonig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.57 / 2.39
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 45.052, 87.920, 87.526, 90.00, 105.14, 90.00
R / Rfree (%) 21.2 / 27.9

Other elements in 4jb4:

The structure of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility also contains other interesting chemical elements:

Iron (Fe) 2 atoms

Fluorine Binding Sites:

The binding sites of Fluorine atom in the Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility (pdb code 4jb4). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 2 binding sites of Fluorine where determined in the Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility, PDB code: 4jb4:
Jump to Fluorine binding site number: 1; 2;

Fluorine binding site 1 out of 2 in 4jb4

Go back to Fluorine Binding Sites List in 4jb4
Fluorine binding site 1 out of 2 in the Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F402

b:14.2
occ:1.00
 FE A:HEM401 2.3 16.6 1.0
NE1 A:TRP51 2.7 14.7 1.0
NB A:HEM401 2.8 15.2 1.0
NE A:ARG48 2.8 6.1 0.4
NA A:HEM401 2.8 16.8 1.0
NE2 A:HIS52 3.1 18.2 1.0
ND A:HEM401 3.2 16.0 1.0
NH2 A:ARG48 3.2 2.0 0.4
NC A:HEM401 3.2 17.0 1.0
CZ A:ARG48 3.3 2.0 0.4
CD1 A:TRP51 3.4 17.2 1.0
C1B A:HEM401 3.5 16.3 1.0
C4A A:HEM401 3.5 17.1 1.0
C1A A:HEM401 3.6 14.4 1.0
C4B A:HEM401 3.7 13.3 1.0
CD2 A:HIS52 3.8 18.6 1.0
CD A:ARG48 3.8 8.0 0.4
CHB A:HEM401 3.8 15.9 1.0
CG A:ARG48 3.8 12.9 0.4
C1C A:HEM401 3.8 15.3 1.0
CE2 A:TRP51 3.9 14.7 1.0
C4D A:HEM401 3.9 19.4 1.0
C4C A:HEM401 3.9 18.3 1.0
CHA A:HEM401 4.0 18.4 1.0
C1D A:HEM401 4.1 19.3 1.0
CHC A:HEM401 4.1 12.9 1.0
CE1 A:HIS52 4.2 13.9 1.0
NE2 A:HIS175 4.3 5.1 1.0
CHD A:HEM401 4.3 16.4 1.0
CZ2 A:TRP51 4.4 12.9 1.0
NH1 A:ARG48 4.6 2.0 0.4
C3A A:HEM401 4.6 16.5 1.0
C2A A:HEM401 4.6 18.3 1.0
C2B A:HEM401 4.6 9.7 1.0
C3B A:HEM401 4.7 8.2 1.0
CG A:TRP51 4.7 17.4 1.0
C2C A:HEM401 4.8 16.6 1.0
C3C A:HEM401 4.9 17.6 1.0
CD2 A:TRP51 4.9 16.7 1.0
CG A:HIS52 5.0 17.2 1.0

Fluorine binding site 2 out of 2 in 4jb4

Go back to Fluorine Binding Sites List in 4jb4
Fluorine binding site 2 out of 2 in the Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Expression, Purification, Characterization, and Solution uc(Nmr) Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility within 5.0Å range:
probe atom residue distance (Å) B Occ
C:F402

b:17.8
occ:1.00
 FE C:HEM401 2.4 15.5 1.0
NE C:ARG48 2.6 4.8 0.4
O C:HOH504 2.7 20.6 1.0
NE1 C:TRP51 2.7 18.8 1.0
NE2 C:HIS52 2.8 23.0 1.0
NH2 C:ARG48 3.0 2.0 0.4
NB C:HEM401 3.1 9.9 1.0
NA C:HEM401 3.1 13.4 1.0
CZ C:ARG48 3.2 2.0 0.4
ND C:HEM401 3.2 13.4 1.0
CD1 C:TRP51 3.3 16.6 1.0
NC C:HEM401 3.4 10.9 1.0
CD2 C:HIS52 3.4 23.4 1.0
CG C:ARG48 3.6 12.6 0.4
CD C:ARG48 3.7 5.8 0.4
C4A C:HEM401 3.7 13.7 1.0
C1B C:HEM401 3.7 12.3 1.0
C1A C:HEM401 3.8 15.8 1.0
CE1 C:HIS52 3.8 22.4 1.0
CHB C:HEM401 3.9 13.1 1.0
C4B C:HEM401 3.9 12.0 1.0
CE2 C:TRP51 4.0 16.9 1.0
C4D C:HEM401 4.0 15.8 1.0
C1D C:HEM401 4.0 16.7 1.0
C4C C:HEM401 4.0 14.8 1.0
C1C C:HEM401 4.1 8.2 1.0
CHA C:HEM401 4.2 14.8 1.0
CHD C:HEM401 4.3 14.1 1.0
CHC C:HEM401 4.4 7.5 1.0
NH1 C:ARG48 4.5 2.0 0.4
CG C:HIS52 4.5 21.6 1.0
CZ2 C:TRP51 4.6 16.3 1.0
CG C:TRP51 4.6 18.0 1.0
C3A C:HEM401 4.6 17.1 1.0
NE2 C:HIS175 4.7 9.1 1.0
C2A C:HEM401 4.7 19.5 1.0
ND1 C:HIS52 4.7 17.2 1.0
C2B C:HEM401 4.8 7.5 1.0
C3B C:HEM401 4.9 10.0 1.0
CD2 C:TRP51 5.0 17.1 1.0

Reference:

A.N.Volkov, A.Wohlkonig, S.H.Soror, N.A.Van Nuland. Expression, Purification, Characterization, and Solution Nuclear Magnetic Resonance Study of Highly Deuterated Yeast Cytochrome C Peroxidase with Enhanced Solubility. Biochemistry V. 52 2165 2013.
ISSN: ISSN 0006-2960
PubMed: 23517193
DOI: 10.1021/BI400220W
Page generated: Thu Aug 1 02:49:33 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy