Atomistry » Fluorine » PDB 4lvt-4mm8 » 4mih
Atomistry »
  Fluorine »
    PDB 4lvt-4mm8 »
      4mih »

Fluorine in PDB 4mih: Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant

Enzymatic activity of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant

All present enzymatic activity of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant:
1.1.3.10;

Protein crystallography data

The structure of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant, PDB code: 4mih was solved by N.Hassan, T.C.Tan, O.Spadiut, I.Pisanelli, L.Fusco, D.Haltrich, C.Peterbauer, C.Divne, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.56 / 2.40
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 110.100, 167.099, 168.472, 90.00, 93.98, 90.00
R / Rfree (%) 20.5 / 26.5

Fluorine Binding Sites:

The binding sites of Fluorine atom in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant (pdb code 4mih). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total 8 binding sites of Fluorine where determined in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant, PDB code: 4mih:
Jump to Fluorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Fluorine binding site 1 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 1 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:F802

b:8.2
occ:1.00
 F3 A:G3F802 0.0 8.2 1.0
C3 A:G3F802 1.3 10.1 1.0
C2 A:G3F802 2.3 12.2 1.0
C4 A:G3F802 2.4 19.1 1.0
O2 A:G3F802 2.6 15.3 1.0
O4 A:G3F802 2.8 26.6 1.0
O4 A:FAD801 3.2 10.7 1.0
ND2 A:ASN596 3.3 20.1 1.0
NE2 A:GLN454 3.4 29.9 1.0
OE1 A:GLN454 3.4 18.0 1.0
C1 A:G3F802 3.6 16.3 1.0
C5 A:G3F802 3.6 22.3 1.0
CD A:GLN454 3.8 21.4 1.0
C4 A:FAD801 3.8 4.3 1.0
CB A:THR160 3.9 11.7 1.0
CG2 A:THR160 3.9 24.6 1.0
O5 A:G3F802 3.9 22.2 1.0
N5 A:FAD801 4.0 10.0 1.0
OD2 A:ASP458 4.1 28.2 1.0
C4X A:FAD801 4.2 2.0 1.0
OG1 A:THR160 4.3 25.3 1.0
CB A:ALA162 4.5 13.4 1.0
CZ A:PHE480 4.5 10.4 1.0
O1 A:G3F802 4.6 18.4 1.0
CG A:ASN596 4.6 19.7 1.0
N3 A:FAD801 4.7 4.6 1.0
C6 A:G3F802 4.9 14.8 1.0
C5X A:FAD801 5.0 12.7 1.0

Fluorine binding site 2 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 2 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 2 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:F802

b:25.1
occ:1.00
 F3 B:G3F802 0.0 25.1 1.0
C3 B:G3F802 1.3 27.0 1.0
C2 B:G3F802 2.3 23.2 1.0
C4 B:G3F802 2.4 22.0 1.0
O2 B:G3F802 2.7 24.7 1.0
O4 B:G3F802 2.9 18.8 1.0
NE2 B:GLN454 3.2 32.2 1.0
O4 B:FAD801 3.3 20.4 1.0
ND2 B:ASN596 3.4 24.3 1.0
CB B:THR160 3.4 16.9 1.0
CG2 B:THR160 3.4 17.8 1.0
C1 B:G3F802 3.6 26.8 1.0
C5 B:G3F802 3.7 19.3 1.0
OG1 B:THR160 3.7 31.0 1.0
OE1 B:GLN454 3.7 33.5 1.0
CD B:GLN454 3.9 32.3 1.0
C4 B:FAD801 3.9 12.8 1.0
O5 B:G3F802 4.0 24.6 1.0
N5 B:FAD801 4.0 19.1 1.0
OD2 B:ASP458 4.2 34.6 1.0
C4X B:FAD801 4.2 16.6 1.0
O1 B:G3F802 4.5 35.5 1.0
CB B:ALA162 4.6 26.5 1.0
CZ B:PHE480 4.6 12.3 1.0
CG B:ASN596 4.7 25.2 1.0
C6 B:G3F802 4.8 24.1 1.0
N3 B:FAD801 4.8 25.1 1.0
CA B:THR160 4.9 20.1 1.0

Fluorine binding site 3 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 3 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 3 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:F802

b:36.9
occ:1.00
 F3 C:G3F802 0.0 36.9 1.0
C3 C:G3F802 1.3 39.5 1.0
C2 C:G3F802 2.3 36.0 1.0
C4 C:G3F802 2.4 33.0 1.0
O2 C:G3F802 2.7 25.5 1.0
O4 C:G3F802 2.9 24.1 1.0
ND2 C:ASN596 3.2 28.1 1.0
O4 C:FAD801 3.2 23.5 1.0
OE1 C:GLN454 3.2 25.0 1.0
CG2 C:THR160 3.3 30.2 1.0
NE2 C:GLN454 3.5 35.4 1.0
C1 C:G3F802 3.6 37.0 1.0
C5 C:G3F802 3.6 31.4 1.0
C4 C:FAD801 3.7 22.6 1.0
CD C:GLN454 3.8 35.5 1.0
CB C:THR160 3.8 44.4 1.0
O5 C:G3F802 4.0 33.1 1.0
N5 C:FAD801 4.1 20.3 1.0
C4X C:FAD801 4.1 20.1 1.0
CB C:ALA162 4.4 40.5 1.0
CG C:ASN596 4.4 35.1 1.0
N3 C:FAD801 4.5 23.2 1.0
OG1 C:THR160 4.5 36.1 1.0
OD2 C:ASP458 4.6 23.4 1.0
CZ C:PHE480 4.6 22.7 1.0
O1 C:G3F802 4.7 40.0 1.0
C6 C:G3F802 4.8 27.0 1.0
CB C:ASN596 4.9 28.1 1.0

Fluorine binding site 4 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 4 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 4 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
D:F802

b:43.3
occ:1.00
 F3 D:G3F802 0.0 43.3 1.0
C3 D:G3F802 1.3 46.8 1.0
C4 D:G3F802 2.3 40.5 1.0
C2 D:G3F802 2.3 41.7 1.0
O4 D:G3F802 2.7 37.9 1.0
O2 D:G3F802 2.8 32.9 1.0
OE1 D:GLN454 3.0 33.2 1.0
NE2 D:GLN454 3.1 40.4 1.0
ND2 D:ASN596 3.1 29.8 1.0
CG2 D:THR160 3.3 37.4 1.0
CD D:GLN454 3.4 36.8 1.0
C5 D:G3F802 3.6 29.0 1.0
C1 D:G3F802 3.6 37.4 1.0
O4 D:FAD801 3.7 34.2 1.0
O5 D:G3F802 4.0 30.3 1.0
CZ D:PHE480 4.1 27.7 1.0
C4 D:FAD801 4.3 37.7 1.0
CG D:ASN596 4.5 30.8 1.0
OD2 D:ASP458 4.5 40.6 1.0
N5 D:FAD801 4.5 35.5 1.0
CE1 D:PHE480 4.6 28.6 1.0
CB D:ALA162 4.6 38.3 1.0
CB D:THR160 4.7 37.2 1.0
C4X D:FAD801 4.7 32.9 1.0
O1 D:G3F802 4.7 35.5 1.0
C6 D:G3F802 4.7 25.8 1.0
CG D:GLN454 4.8 35.8 1.0
CE2 D:PHE480 4.8 25.0 1.0
NE2 D:HIS456 5.0 35.2 1.0

Fluorine binding site 5 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 5 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 5 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
E:F802

b:17.0
occ:1.00
 F3 E:G3F802 0.0 17.0 1.0
C3 E:G3F802 1.3 20.5 1.0
C2 E:G3F802 2.3 20.0 1.0
C4 E:G3F802 2.3 19.4 1.0
O2 E:G3F802 2.7 20.0 1.0
O4 E:G3F802 2.8 16.0 1.0
NE2 E:GLN454 3.1 19.3 1.0
ND2 E:ASN596 3.2 12.7 1.0
O4 E:FAD801 3.3 19.5 1.0
OE1 E:GLN454 3.4 20.2 1.0
C1 E:G3F802 3.6 24.8 1.0
C5 E:G3F802 3.6 18.9 1.0
CD E:GLN454 3.7 17.0 1.0
C4 E:FAD801 3.9 14.0 1.0
CB E:THR160 3.9 30.8 1.0
O5 E:G3F802 4.0 20.2 1.0
OG1 E:THR160 4.0 30.5 1.0
CB E:ALA162 4.1 21.1 1.0
N5 E:FAD801 4.1 24.9 1.0
OD2 E:ASP458 4.2 18.2 1.0
C4X E:FAD801 4.3 16.2 1.0
CG2 E:THR160 4.3 37.2 1.0
CG E:ASN596 4.4 15.7 1.0
CZ E:PHE480 4.6 14.6 1.0
O1 E:G3F802 4.6 35.4 1.0
N3 E:FAD801 4.7 18.4 1.0
C6 E:G3F802 4.8 18.4 1.0

Fluorine binding site 6 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 6 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 6 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
F:F802

b:41.1
occ:1.00
 F3 F:G3F802 0.0 41.1 1.0
C3 F:G3F802 1.3 41.2 1.0
C2 F:G3F802 2.3 38.2 1.0
C4 F:G3F802 2.3 33.2 1.0
O2 F:G3F802 2.8 34.6 1.0
O4 F:G3F802 3.0 23.4 1.0
O4 F:FAD801 3.0 27.6 1.0
ND2 F:ASN596 3.4 30.3 1.0
CB F:THR160 3.5 25.1 1.0
NE2 F:GLN454 3.6 27.5 1.0
CG2 F:THR160 3.6 20.3 1.0
C1 F:G3F802 3.6 33.3 1.0
C5 F:G3F802 3.6 35.5 1.0
C4 F:FAD801 3.6 25.3 1.0
OG1 F:THR160 3.8 19.8 1.0
N5 F:FAD801 3.9 30.0 1.0
O5 F:G3F802 3.9 36.8 1.0
C4X F:FAD801 4.0 27.1 1.0
OD2 F:ASP458 4.1 37.4 1.0
OE1 F:GLN454 4.2 30.1 1.0
CD F:GLN454 4.3 27.6 1.0
CB F:ALA162 4.4 22.1 1.0
N3 F:FAD801 4.5 25.3 1.0
O1 F:G3F802 4.7 27.2 1.0
CG F:ASN596 4.7 27.0 1.0
C6 F:G3F802 4.7 36.0 1.0
CZ F:PHE480 4.8 18.0 1.0
CA F:THR160 4.8 24.6 1.0
C5X F:FAD801 4.9 25.6 1.0

Fluorine binding site 7 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 7 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 7 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
G:F802

b:30.1
occ:1.00
 F3 G:G3F802 0.0 30.1 1.0
C3 G:G3F802 1.3 34.1 1.0
C2 G:G3F802 2.3 35.8 1.0
C4 G:G3F802 2.3 35.3 1.0
O2 G:G3F802 2.7 35.5 1.0
O4 G:G3F802 2.7 40.8 1.0
NE2 G:GLN454 3.3 33.5 1.0
ND2 G:ASN596 3.3 29.0 1.0
O4 G:FAD801 3.4 33.0 1.0
CG2 G:THR160 3.4 27.1 1.0
OE1 G:GLN454 3.5 27.3 1.0
C1 G:G3F802 3.6 33.5 1.0
C5 G:G3F802 3.6 32.2 1.0
CB G:THR160 3.8 19.0 1.0
CD G:GLN454 3.8 33.3 1.0
OD2 G:ASP458 3.8 31.0 1.0
O5 G:G3F802 4.0 32.2 1.0
C4 G:FAD801 4.0 27.2 1.0
OG1 G:THR160 4.2 34.6 1.0
N5 G:FAD801 4.2 29.0 1.0
C4X G:FAD801 4.4 31.1 1.0
CZ G:PHE480 4.5 18.0 1.0
CB G:ALA162 4.5 20.2 1.0
O1 G:G3F802 4.5 37.2 1.0
CG G:ASN596 4.6 24.2 1.0
C6 G:G3F802 4.8 33.1 1.0
N3 G:FAD801 4.9 23.4 1.0
CG G:ASP458 5.0 29.3 1.0

Fluorine binding site 8 out of 8 in 4mih

Go back to Fluorine Binding Sites List in 4mih
Fluorine binding site 8 out of 8 in the Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 8 of Pyranose 2-Oxidase From Phanerochaete Chrysosporium, Recombinant H158A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
H:F802

b:31.1
occ:1.00
 F3 H:G3F802 0.0 31.1 1.0
C3 H:G3F802 1.3 31.2 1.0
C2 H:G3F802 2.3 31.9 1.0
C4 H:G3F802 2.3 27.5 1.0
O4 H:G3F802 2.8 24.0 1.0
O2 H:G3F802 2.8 32.2 1.0
ND2 H:ASN596 3.1 37.9 1.0
O4 H:FAD801 3.2 35.9 1.0
OE1 H:GLN454 3.2 34.3 1.0
NE2 H:GLN454 3.3 43.0 1.0
C1 H:G3F802 3.6 29.3 1.0
C5 H:G3F802 3.6 32.1 1.0
CD H:GLN454 3.7 36.8 1.0
CB H:THR160 3.7 29.4 1.0
C4 H:FAD801 3.8 31.0 1.0
CG2 H:THR160 3.8 31.9 1.0
O5 H:G3F802 4.0 27.9 1.0
OD2 H:ASP458 4.1 36.9 1.0
N5 H:FAD801 4.1 24.1 1.0
OG1 H:THR160 4.1 32.7 1.0
C4X H:FAD801 4.2 22.6 1.0
CG H:ASN596 4.4 41.2 1.0
CB H:ALA162 4.4 36.7 1.0
CZ H:PHE480 4.6 31.8 1.0
O1 H:G3F802 4.6 24.4 1.0
N3 H:FAD801 4.6 29.7 1.0
C6 H:G3F802 4.8 35.6 1.0

Reference:

N.Hassan, T.C.Tan, O.Spadiut, I.Pisanelli, L.Fusco, D.Haltrich, C.K.Peterbauer, C.Divne. Crystal Structures of Phanerochaete Chrysosporium Pyranose 2-Oxidase Suggest That the N-Terminus Acts As A Propeptide That Assists in Homotetramer Assembly. Febs Open Bio V. 3 496 2013.
ISSN: ESSN 2211-5463
PubMed: 24282677
DOI: 10.1016/J.FOB.2013.10.010
Page generated: Thu Aug 1 03:44:48 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy