Fluorine in PDB 5f9t: Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota

All present enzymatic activity of Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota:
2.7.7.7;

The structure of Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota, PDB code: 5f9t was solved by L.Zhao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	84.26 / 2.05
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	97.298, 97.298, 202.906, 90.00, 90.00, 120.00
R / Rfree (%)	21.4 / 26.1

The binding sites of Fluorine atom in the Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota (pdb code 5f9t). This binding sites where shown within 5.0 Angstroms radius around Fluorine atom.
In total only one binding site of Fluorine was determined in the Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota, PDB code: 5f9t:

Fluorine binding site 1 out of 1 in the Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota


Mono view


Stereo pair view

A full contact list of Fluorine with other atoms in the F binding site number 1 of Base Pairing Mechanism of N2,3-Ethenoguanine with Dctp By Human Polymerase Iota within 5.0Å range: probe atom residue distance (Å) B Occ C:F840 b:43.6 occ:1.00 F C:EFG840 0.0 43.6 1.0 C2' C:EFG840 1.4 42.4 1.0 C3' C:EFG840 2.2 44.3 1.0 C1' C:EFG840 2.3 44.7 1.0 O4' C:EFG840 2.7 47.3 1.0 N9 C:EFG840 2.8 45.7 1.0 C9 C:EFG840 2.8 58.5 1.0 C8 C:DG841 2.9 37.0 1.0 C4' C:EFG840 3.0 48.4 1.0 N3 C:EFG840 3.1 54.8 1.0 C4 C:EFG840 3.1 47.2 1.0 O C:HOH914 3.2 51.2 1.0 N7 C:DG841 3.2 38.5 1.0 O A:HOH652 3.3 43.6 1.0 O3' C:EFG840 3.4 40.9 1.0 C10 C:EFG840 3.8 61.2 1.0 C8 C:EFG840 3.8 44.6 1.0 N9 C:DG841 3.9 36.9 1.0 C5' C:EFG840 3.9 51.5 1.0 O5' C:EFG840 4.0 64.2 1.0 O5' C:DG841 4.0 43.1 1.0 OP2 C:DG841 4.2 47.7 1.0 C2 C:EFG840 4.2 59.5 1.0 P C:DG841 4.2 46.8 1.0 C5 C:EFG840 4.2 46.8 1.0 C5 C:DG841 4.3 39.0 1.0 O4' C:DG841 4.3 36.5 1.0 C1' C:DG841 4.5 36.2 1.0 N2 C:EFG840 4.6 64.3 1.0 N7 C:EFG840 4.6 44.9 1.0 C4 C:DG841 4.6 36.6 1.0 P C:EFG840 4.7 68.5 1.0 OE2 A:GLU305 4.7 48.5 1.0 C2' C:DG841 4.8 34.7 1.0 OE1 A:GLN59 5.0 44.9 1.0 C5' C:DG841 5.0 39.0 1.0

L.Zhao, M.G.Pence, P.P.Christov, Z.Wawrzak, J.Y.Choi, C.J.Rizzo, M.Egli, F.P.Guengerich. Basis of Miscoding of the Dna Adduct N2,3-Ethenoguanine By Human Y-Family Dna Polymerases. J.Biol.Chem. 2012.
ISSN: ESSN 1083-351X
PubMed: 22910910
DOI: 10.1074/JBC.M112.403253